Treponema pallidum (Syphilis) Antibody with Reflex to Rapid Plasma Reagin (RPR)

Synonyms

  • LAB3013
  • REVERSE ALGORITHM
  • SYPHILIS SCREEN
  • TREPONEMA PALLIDUM (SYPHILIS) ANTIBODY W/REFLEX

Cerner Name

Syphilis Screen

Clinical Info

This test is the first step of the Reverse Syphilis Screening algorithm. Positive Treponema pallidum antibody test results will reflex to RPR for confirmation. Discordant samples will be tested with an alternative Treponemal assay.

The Syphilis Screen should not be used for infants <30 days to diagnose congenital syphilis, as maternal antibodies are present. A RPR (Syphilis Titer) is the appropriate test. The Syphilis Screen will be canceled and RPR (Syphilis titer) ordered if patient is <30 days old. 

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous

Specimen Types

Blood

Specimen Volume

1 mL of serum

Container

Gold Top Tube

Collection Instructions

Container/Tube: Gold-top tube(s)
Specimen: 1 mL of serum
Transport Temperature: Refrigerate

Specimen Storage:
Specimens must be centrifuged and refrigerated after draw to maintain stability/integrity Syphilis specimen should be kept refrigerated between 2-8° Centigrade/Celsius.
Stability: 7 Days Refrigerated

Transport Instructions

Refrigerate

Specimen Stability

7 Days Refrigerated

Methodology

Chemiluminescent  Immunoassay
This test is for screening Syphilis with equivocal or positive reflex to RPR

Days Performed

Monday through Sunday, Continuously

Performing Laboratories

Northwell Health Laboratories

CPT

86780

PDM

5700700

Results
Component Name Base Name Common Name External Name
TREPONEMA PALLIDUM AB CLIA TPPA TREPONEMA PALLIDUM AB CLIA Treponema pallidum Antibody Screen

Result Interpretation

< 0.90 Index -   Negative 
No serological evidence of infection with Treponema pallidum (incubating or early primary syphilis cannot be excluded).
0.90 to 1.09 Index-   Equivocal 
The equivocal sample should be repeat tested. Samples which are negative after a second testing should be considered negative. Samples which are positive at the second testing should be considered positive. In case the result remains in this range after repeat  testing, a second sample should be collected and tested no less than one or two weeks later
≥1.10 Index-  Positive
 Presence of detectable Treponema pallidum total antibodies

Forms
ID Serology LAB3013 build edit