HLA B27 Molecular Detection Build info

Synonyms	HLA B27 GENOTYPING ANKYLOSING SPONDYLITIS HLA-B27 ANTIGEN HLX HLA B27 GENOTYPE CASE HLA ANTIGEN B27 LAB11402
Short Name	HLA B27 GENOTYPING
Procedure Master Number	LAB11402
Procedure ID	115145
Clinical Info	Ankylosing spondylitis (AS) is a rheumatic inflammatory disease that belongs to the group of spondyloarthropathies. Human leukocyte antigen B27 (HLA-B27) is strongly associated with AS. There has been an increasing appreciation of HLA-B27 positivity in facilitating the diagnosis of an early stage of AS. About 90% of AS patients are HLA-B27 positive, compared to 5-10% of the general population. In addition, an increased prevalence of HLA-B27 has been reported in patients with Reiter's syndrome, anterior uveitis, psoriatic arthritis, and inflammatory bowel disease. As a result, tests of HLA-B27 are a valuable adjunct in the diagnosis of these diseases. Limitations: This test does not rule out the B*27:06 and 27:09 alleles, which are not associated with spondyloarthropathies. Certain rare alleles present in less than 1 percent of the population will not be detected. Other rare, or uncharacterized alleles may occur which may lead to false positive or false negative results.
Specimen Type	Blood
Container	Lavender Top Tube
Collection Instructions	Container/Tube: Lavender Top EDTA tube Specimen: 1-3 mL peripheral blood Transport Temperature: Room Temperature Specimens should be accompanied by a test request form and a signed consent form stating that the patient agrees to be tested for HLA-B27 genotyping. The consent form should be signed by the patient or designee and the health care provider.
Transport Instructions	Room temperature
Specimen Stability	Specimens are stable for a week refrigerated.
Methodology	Real Time Polymerase Chain Reaction (RT-PCR)
Days Performed	Monday through Friday TAT: 8 Calendar days
Performing Laboratory	Northwell Health Laboratories
CPT	81374
PDM	238466
Results