HLX HLA B27 Genotype Case HLX HLA B27 Genotype Case

Synonyms

Allscripts (AEHR) Order Name

HLA B27 Genotyping

Sunrise Clinical Manager (SCM) Order Name

HLA B27 Genotyping

Clinical Info

Ankylosing spondylitis (AS) is a rheumatic inflammatory disease that belongs to the group of spondyloarthropathies. Human leukocyte antigen B27 (HLA-B27) is strongly associated with AS. There has been an increasing appreciation of HLA-B27 positivity in facilitating the diagnosis of an early stage of AS. About 90% of AS patients are HLA-B27 positive, compared to 5-10% of the general population. In addition, an increased prevalence of HLA-B27 has been reported in patients with Reiter's syndrome, anterior uveitis, psoriatic arthritis, and inflammatory bowel disease. As a result, tests of HLA-B27 are a valuable adjunct in the diagnosis of these diseases.
Limitations: This test does not rule out the B*27:06 and 27:09 alleles, which are not associated with spondyloarthropathies. Certain rare alleles present in less than 1 percent of the population will not be detected. Other rare, or uncharacterized alleles may occur which may lead to false positive or false negative results.

Specimen Type

Blood

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender Top EDTA tube 
Specimen: 1-3 mL peripheral blood
Transport Temperature: Room Temperature
Specimens should be accompanied by a test request form and a signed consent form stating that the patient agrees to be tested for HLA-B*5701 genotyping. The consent form should be signed by the patient or designee and the health care provider. 

Transport Instructions

Room temperature

Specimen Stability

Specimens are stable for a week refrigerated.

Methodology

Real Time Polymerase Chain Reaction (RT-PCR)

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

81374

PDM

238466

Result Interpretation

An interpretative report will be provided

Forms

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