Joint PCR Panel Joint PCR

Synonyms

Joint Infection, Infected Joint, Synovial

Allscripts (AEHR) Order Name

Joint PCR Panel

Sunrise Clinical Manager (SCM) Order Name

Joint PCR Panel

Clinical Info


This is a multiplex PCR which detects the following organisms/resistance genes in infected joint fluid: Anaerococcus prevotii/vaginalis, Clostridium perfringens, 
Cutibacterium avidum/granulosum, Enterococcus faecalis (including VRE), Enterococcus faecium (including VRE), Finegoldia magna, Parvimonas micra, Peptoniphilus spp., 
Peptostreptococcus anaerobius, Staphylococcus aureus (including MRSA), Staphylococcus lugdunensis, Streptococcus pyogenes (GAS), Streptococcus agalactiae (GBS), 
Streptococcus pneumoniae, other Streptococcus species at the genus level, Bacteroides fragilis, Citrobacter spp., Enterobacter cloacae complex, Escherichia coli, 
Haemophilus influenzae, Kingella kingae, Klebsiella aerogenes, Klebsiella pneumoniae group, Morganella morganii, Neisseria gonorrhoeae, Proteus spp., Pseudomonas 
aeruginosa, Salmonella spp., Serratia marcescens, Candida albicans, other Candida species at the genus level, Carbapenemases (KPC, OXA-48-like, NDM, VIM, IMP) and
Extended Spectrum Beta-Lactamases (CTX-M).
Please indicate Specimen Type/Source (Knee, hip, etc) when ordering.  The Joint PCR Panel is intended as an aid to diagnosis of infected joint.  The assay detects 
multiple bacteria and yeast organisms, and should be utilized in conjunction with a Joint Culture.  Lab results should interpreted in the context of the patient's clinical 
presentation, history, and other tests.      

Specimen Type

Body Fluid, Other

Container

Sterile

Collection Instructions

Sterilely transfer at least 0.5 mL synovial fluid into a sterile screw top container or tube. This may be combined with sample for Joint Culture.  
But should be sent separately from other other synovial fluid tests such as cell count, etc. .  

Transport Instructions

Refrigerate (2-8 C)

Specimen Stability

Refrigerate (2-8 C) 3 days; Frozen (-20 C or lower) for longer storage

Methodology

Mutliplex Nucleic Acid Amplification by Polymerase Chain Reaction

Days Performed

Monday to Sunday

Performing Laboratory

Northwell Health Laboratories-Little Neck Parkway

CPT

PDM

237266

Result Interpretation

Please indicate Specimen Type/Source (Knee, hip, etc) when ordering.  Assays results should always interpreted in the context of the patient's clinical presentation, history, and other tests.  Detected targets may represent nonviable organisms or skin flora.  Panel targets do not include Staphylococcus epidermidis, other coagulase negative Staphylococci, or CutibacteriumJoint Culture is recommended in conjunction with Joint PCR to help identify organisms absent from panel and for antibiotic susceptiblity testing.        

Table of Joint Panel Targets

Gram Positive Bacteria Gram Negative Bacteria Yeast
Anaerococcus prevotii/vaginalis  Bacteroides fragilis  Candida
Clostridium perfringens  Citrobacter  Candida albicans
Cutibacterium avidum/granulosum  Enterobacter cloacae complex   
Enterococcus faecalis  Escherichia coli  Resistance Genes
Enterococcus faecium  Haemophilus influenzae  CTX-M 
Finegoldia magna  Kingella kingae  KPC 
Parvimonas micra  Klebsiella aerogenes  NDM 
Peptoniphilus  Klebsiella pneumoniae group  vanA/B
Peptostreptococcus anaerobius  Morganella morganii  IMP 
Staphylococcus aureus Neisseria gonorrhoeae mecA/C and MREJ (MRSA) 
Staphylococcus lugdunensis Proteus spp. OXA-48-like 
Streptococcus spp. Pseudomonas aeruginosa VIM
Streptococcus pyogenes Salmonella spp.  
Streptococcus agalactiae Serratia marcescens  
Streptococcus pneumoniae    

Forms


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