This is a multiplex PCR which detects the following organisms/resistance genes in infected joint fluid: Anaerococcus prevotii/vaginalis, Clostridium perfringens, Cutibacterium avidum/granulosum, Enterococcus faecalis (including VRE), Enterococcus faecium (including VRE), Finegoldia magna, Parvimonas micra, Peptoniphilus spp., Peptostreptococcus anaerobius, Staphylococcus aureus (including MRSA), Staphylococcus lugdunensis, Streptococcus pyogenes (GAS), Streptococcus agalactiae (GBS), Streptococcus pneumoniae, other Streptococcus species at the genus level, Bacteroides fragilis, Citrobacter spp., Enterobacter cloacae complex, Escherichia coli, Haemophilus influenzae, Kingella kingae, Klebsiella aerogenes, Klebsiella pneumoniae group, Morganella morganii, Neisseria gonorrhoeae, Proteus spp., Pseudomonas aeruginosa, Salmonella spp., Serratia marcescens, Candida albicans, other Candida species at the genus level, Carbapenemases (KPC, OXA-48-like, NDM, VIM, IMP) and Extended Spectrum Beta-Lactamases (CTX-M). Please indicate Specimen Type/Source (Knee, hip, etc) when ordering. The Joint PCR Panel is intended as an aid to diagnosis of infected joint. The assay detects multiple bacteria and yeast organisms, and should be utilized in conjunction with a Joint Culture. Lab results should interpreted in the context of the patient's clinical presentation, history, and other tests.
Sterilely transfer at least 0.5 mL synovial fluid into a sterile screw top container or tube. This may be combined with sample for Joint Culture. But should be sent separately from other other synovial fluid tests such as cell count, etc. .
Transport Instructions
Refrigerate (2-8 C)
Specimen Stability
Refrigerate (2-8 C) 3 days; Frozen (-20 C or lower) for longer storage
Methodology
Mutliplex Nucleic Acid Amplification by Polymerase Chain Reaction
