aHUS Complement Panel, S and P AHUSD

Synonyms
Allscripts (AEHR) Order Name	aHUS Complement Panel, S and P
Sunrise Clinical Manager (SCM) Order Name	Atypical Hemolytic Uremic Syndrome Complement P
EPIC Order Name	Atypical Hemolytic Uremic Syndrome (AHUS) Complement Panel
Clinical Info	Detecting deficiencies in the alternative pathway that can cause atypical-hemolytic uremic syndrome, dense deposit disease, and C3 glomerulonephritis ORDERING GUIDANCE This test should be performed prior to treatment initiation and in the absence of therapy with complement inhibitors, such as eculizumab or ravulizumab. Complement inhibitors will affect performance of these assays. For evaluating patients with possible thrombotic microangiopathies (TMA), the recommended first-tier test is ADM13 / ADAMTS13 Activity and Inhibitor Profile, Plasma. This test should be a second-tier test for TMA. For patients who have received eculizumab or need to monitor response to eculizumab therapy, the recommended test is ECUMP / Eculizumab Monitoring Panel, Serum. Soluble membrane attack complex (sMAC) should not be used as a standalone assay to monitor eculizumab efficiency.
Specimen Type	Blood
Specimen Volume
Container	Lavender Top Tube
Collection Instructions	Serum and Plasma are required for this test Container/Tube: Both Lavender Top AND Red Top Tube Specimen: 1.5 mL EDTA plasma ( 1.5 mL min) AND 1.5 mL serum (1.5 mL min) Collection Instructions: 1. Immediately after specimen collection, place the tube on wet ice. 2. Centrifuge; 1500 x g for 10 minutes at 4 degrees C and aliquot plasma from Lavender tube and serum from the Red tube into plastic vial. 3. Freeze specimen within 30 minutes. Patient Preparation: 1. Fasting preferred. 2. Samples should not be collected earlier than 48 hours following plasma exchange. Alternate Tube: Plasma : Light Blue (3.3 % sodium citrate) Serum Gold Top Tube
Transport Instructions	Frozen
Specimen Stability	Plasma 14 Days Frozen Serum Red 14 Days Frozen
Methodology	Nephelometry Automated Liposome Lysis Assay Enzyme-Linked Immunosorbent Assay (ELISA)
Days Performed	TAT: 13 - 22 Days
Performing Laboratory	Mayo Medical Laboratories
CPT	86160 x 6 86161 86162
PDM	225440
Result Interpretation FACTOR B COMPLEMENT ANTIGEN 15.2-42.3 mg/dL SC5b-9 COMPLEMENT < or =250 ng/mL FACTOR H COMPLEMENT ANTIGEN 18.5 to 40.8 mg/dL CBb COMPLEMENT ACTIVATION FRAGMENT < or =1.6 mcg/mL COMPLEMENT C4 14-40 mg/dL COMPLEMENT C3 75-175 mg/dL ALTERNATIVE COMPLEMENT, PATHWAY (AH50) FUNCTIONAL > or =46% Normal COMPLEMENT, TOTAL 30-75 U/mL
Forms