Full T Cell Subset FTHS

Synonyms

Allscripts (AEHR) Order Name

Full T Cell Subset

Sunrise Clinical Manager (SCM) Order Name

Full T Cell Subset

EPIC Order Name

Lymphocyte Subsets: T, B and Natural Killer (NK) cells

Clinical Info

This assay provides absolute cell counts and percentages for CD3+ T-cells, CD4 and CD8 T cell subsets, CD19+ B-cells, CD16/CD56 natural killer cells,as well as CD4/CD8 ratio.

Specimen Type

Blood

Container

Lavender-top (EDTA) tube or Green (Sodium Heparin)

Collection Instructions

Specimen must arrive within 12 hours of draw. Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s).
 
Container/Tube: Lavender-top (EDTA) tube or Green (Sodium Heparin) Tube
Specimen:  3 mL of  whole blood
Collection Instructions:  Forward promptly. Specimen cannot be refrigerated.
Rejection Criteria - All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 24 hours post collection and the sample has not been preserved, unless viability is greater than 50%.

Transport Instructions

Ambient

Specimen Stability

Delivery to the lab within 12 hours is recommended

Methodology

Flow Cytometry
Includes CD3, CD4, CD8, CD16/56, CD19, ABS CD3, ABS CD4, ABS CD8, ABS CD16/56, ABS CD19 AND 4/8 RATIO.

Days Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays  from 8AM -4PM. Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Performing Laboratory

Northwell Health Laboratories at CFAM

CPT

86359
86360
86355
86357

PDM

5201201

Result Interpretation

	CD3	CD4	CD8	CD19	CD1656	ABS CD3	ABS CD4	ABS CD8	ABS CD19	ABS CD1656	4/8 Ratio
Age	%	%	%	%	%	cells/μl	cells/μl	cells/μl	cells/μl	cells/μl
0-3 mo	53-84	35-64	12-28	6-32	4-18	2500-5500	1600-4000	560-1700	300-2000	170-1110	NA
3-6 mo	51-77	35-56	12-23	11-41	3-14	2500-5600	1800-4000	590-1600	430-3000	170-830	NA
6-12 mo	49-76	31-56	12-24	14-37	3-15	1900-5900	1400-4300	500-1700	610-2600	160-950	NA
1-2 yr	53-75	32-51	14-30	16-35	3-15	2100-6200	1300-3400	620-2000	720-2600	180-920	NA
2-6 yr	56-75	28-47	16-30	14-33	4-17	1400-3700	700-2200	490-1300	390-1400	130-720	NA
6-12 yr	60-76	31-47	18-35	13-27	4-17	1200-2600	650-1500	370-1100	270-860	110-480	NA
12-18 yr	56-84	31-52	18-35	6-23	3-22	1000-2200	530-1300	330-920	110-570	70-480	NA
18-66 yr	59-83	30-62	12-36	6-24	5-23	672-1870	489-1457	142-740	84-469	71-410	0.90-3.60
66-150 yr	58-84	30-56	11-43	4-18	7-27	396-2024	325-1251	90-775	32-326	80-426	0.86-4.14

Reference ranges for pediatric population (0-18 years old) are from Shearer, William T., et al. "Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study." Journal of Allergy and Clinical Immunology 112.5 (2003): 973-980.

Reference ranges for adult (18-66 years old) and geriatric (66 years old and above) populations were developed at North Shore University Hospital at Manhasset, New York.

Forms

• Heme/Onc Requisition