Lymphocyte Subsets: T, B and Natural Killer (NK) cells Build info

Synonyms

  • LYMPHOCYTE SUBSETS: T, B AND NATURAL KILLER (NK) CELLS
  • CD56
  • CD3
  • T-CELL
  • CD19
  • NK-CELL
  • CD8
  • FTHS
  • CD4
  • CD16
  • Full T-cell subset
  • LAB10334

Short Name

LYMPHOCYTE SUBSETS: T, B AND NATURAL KILLER (NK) CELLS

Procedure Master Number

LAB10334

Procedure ID

114014

Clinical Info

This assay provides absolute cell counts and percentages for CD3+ T-cells, CD4 and CD8 T cell subsets, CD19+ B-cells, CD16/CD56 natural killer cells,as well as CD4/CD8 ratio.

Specimen Type

Blood

Container

Lavender-top (EDTA) tube or Green (Sodium Heparin)

Collection Instructions

Specimen must arrive within 12 hours of draw. Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s).
 
Container/Tube: Lavender-top (EDTA) tube or Green (Sodium Heparin) Tube
Specimen:  3 mL of  whole blood
Collection Instructions:  Forward promptly. Specimen cannot be refrigerated.
Rejection Criteria - All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 24 hours post collection and the sample has not been preserved, unless viability is greater than 50%.

Transport Instructions

Ambient

Specimen Stability

Delivery to the lab within 12 hours is recommended

Methodology

Flow Cytometry
Includes CD3, CD4, CD8, CD16/56, CD19, ABS CD3, ABS CD4, ABS CD8, ABS CD16/56, ABS CD19 AND 4/8 RATIO.

Days Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays  from 8AM -4PM. Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Performing Laboratory

Northwell Health Laboratories at CFAM

CPT

86359
86360
86355
86357

PDM

5201201

Results
Component Name Base Name Common Name External Name
4/8 RATIO 48RATIO 4 8 RATIO 4/8 Ratio
ABS CD1656 CD1656ABS CD1656 ABS Abs Cd1656
ABS CD19 CD19ABS CD19 ABS
ABS CD3 CD3TABS CD3 ABS CD3 Abs
ABS CD4 CD4ABS CD4 ABS Abs Cd4
ABS CD8 CD8ABS CD8 ABS Abs Cd8
CD1656 % CD1656PCT CD1656 PCT Cd1656 %
CD19 % CD19PCT CD19 PCT Cd19 %
CD3 % CD3PCT CD3 PCT Cd3 %
CD4 % CD4PCT CD4 PCT Cd4 %
CD8 % CD8PCT CD8 PCT Cd8 %
VIABILITY VIABILITY VIABILITY Viability