Inpatient and Outpatient Orderable

Lymphocyte Subsets: T, B and Natural Killer (NK) cells

Synonyms

  • CD16
  • CD19
  • CD3
  • CD4
  • CD56
  • CD8
  • FTHS
  • LAB10334
  • LYMPHOCYTE SUBSETS: T, B AND NATURAL KILLER (NK) CELLS
  • NK-CELL
  • T-CELL

Cerner Name

Full T Cell Subset

Clinical Info

This assay provides absolute cell counts and percentages for CD3+ T-cells, CD4 and CD8 T cell subsets, CD19+ B-cells, CD16/CD56 natural killer cells,as well as CD4/CD8 ratio.

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous

Specimen Types

Blood

Container

Lavender-top (EDTA) tube or Green (Sodium Heparin)

Collection Instructions

Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s).
Forward promptly. Specimen cannot be refrigerated.
Rejection Criteria - All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 24 hours post collection and the viability is less than 50%.

Specimen Volume

3 ml

Transport Instructions

Ambient

Specimen Stability

Delivery to the lab within 12 hours is recommended

Methodology

Flow Cytometry
Includes CD3, CD4, CD8, CD16/56, CD19, ABS CD3, ABS CD4, ABS CD8, ABS CD16/56, ABS CD19 AND 4/8 RATIO.

Days Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays  from 8AM -4PM. Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Performing Laboratory

Northwell Health Laboratories

CPT

86359
86360
86355
86357

PDM

5201201

Only Orderable at Locations:

Orderable Everywhere

Results
Component Name Base Name Common Name External Name
CD3 % CD3PCT CD3 PCT %CD3
ABS CD3 CD3TABS CD3 ABS ABS CD3
CD4 % CD4PCT CD4 PCT % CD4
ABS CD4 CD4ABS CD4 ABS ABSO CD4
CD8 % CD8PCT CD8 PCT %CD8
ABS CD8 CD8ABS CD8 ABS ABS CD8
CD19 % CD19PCT CD19 PCT %CD19
ABS CD19 CD19ABS CD19 ABS ABS CD19
CD1656 % CD1656PCT CD1656 PCT %CD1656
ABS CD1656 CD1656ABS CD1656 ABS ABS CD1656
4/8 RATIO 48RATIO 4 8 RATIO 4/8 Ratio
VIABILITY VIABILITY VIABILITY Viability

Result Interpretation

 

CD3

CD4

CD8

CD19

CD1656

ABS CD3

ABS CD4

ABS CD8

ABS CD19

ABS CD1656

4/8 Ratio

Age

%

%

%

%

%

cells/μl

cells/μl

cells/μl

cells/μl

cells/μl

 

0-3 mo

53-84

35-64

12-28

6-32

4-18

2500-5500

1600-4000

560-1700

300-2000

170-1110

NA

3-6 mo

51-77

35-56

12-23

11-41

3-14

2500-5600

1800-4000

590-1600

430-3000

170-830

NA

6-12 mo

49-76

31-56

12-24

14-37

3-15

1900-5900

1400-4300

500-1700

610-2600

160-950

NA

1-2 yr

53-75

32-51

14-30

16-35

3-15

2100-6200

1300-3400

620-2000

720-2600

180-920

NA

2-6 yr

56-75

28-47

16-30

14-33

4-17

1400-3700

700-2200

490-1300

390-1400

130-720

NA

6-12 yr

60-76

31-47

18-35

13-27

4-17

1200-2600

650-1500

370-1100

270-860

110-480

NA

12-18 yr

56-84

31-52

18-35

6-23

3-22

1000-2200

530-1300

330-920

110-570

70-480

NA

18-66 yr

59-83

30-62

12-36

6-24

5-23

672-1870

489-1457

142-740

84-469

71-410

0.90-3.60

66-150 yr

58-84

30-56

11-43

4-18

7-27

396-2024

325-1251

90-775

32-326

80-426

0.86-4.14

Reference ranges for pediatric population (0-18 years old) are from Shearer, William T., et al. "Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study." Journal of Allergy and Clinical Immunology 112.5 (2003): 973-980.

Reference ranges for adult (18-66 years old) and geriatric (66 years old and above) populations were developed at North Shore University Hospital at Manhasset, New York.

 

Forms
LAB10334 build edit