Insulin Free and Total INSFREE

Synonyms
Allscripts (AEHR) Order Name	Insulin, Free
Sunrise Clinical Manager (SCM) Order Name	Insulin, Free
EPIC Order Name	Insulin, Free and Total
Clinical Info	This test may be useful for diabetic patients treated with insulin analogs, as shown in the table below. Every patient's antibodies are different, and the specificity and avidity of the antibodies may affect the test.
Specimen Type	Blood
Specimen Volume	3 mL serum (1.5 mL min)
Container	Gold Top Tube
Collection Instructions	Container/ Tube: Gold top tube Specimen: 3 mL serum (1.5 mL min) Transport Temperature: Refrigerated Stability: 2 Days Room Temperature 10 Days Refrigerated 200 Days Frozen
Transport Instructions	Refrigerated
Specimen Stability	2 Days Room Temperature 10 Days Refrigerated 200 Days Frozen
Methodology	Chemiluminescent immunometric assay (IMA) after insulin extraction of free and total insulin
Days Performed
Performing Laboratory	Esoterix Inc, Calabasas Hills, CA
CPT	83527-Free Insulin 83525-Total Insulin ;
PDM	5902410
Result Interpretation Insulin, Free, S Pubertal Children and Adults (fasting): 0 - 17 Insulin, Total, S Non-Diabetic: In the absence of insulin-binding antibodies, the free and total insulin assays are equivalent. However, this assay is intended for use in diabetics with insulin autoantibody present. Measurement is performed on acid-treated samples and, therefore, the sensitivity and absolute values by this method may differ from our direct insulin ICMA. Insulin Dependent Diabetic Patients: Free Insulin levels vary depending on the capacity and affinity of circulating insulin-binding antibodies and the dose of insulin given to the patient. Total insulin levels represent free insulin and antibody bound insulin fractions
Forms