Mycoplasma pneumoniae PCR MYCOPCR

Synonyms
Allscripts (AEHR) Order Name	Mycoplasma Pneumoniae PCR
Sunrise Clinical Manager (SCM) Order Name	Mycoplasma pneumoniae PCR
EPIC Order Name	Mycoplasma pneumoniae Molecular Detection
Clinical Info	Diagnosing infections due to Mycoplasma (Mycoplasmoides) pneumoniae Assessing macrolide susceptibility
Specimen Type	Body Fluid, CSF, Swab, Respiratory, Nasal, Nasopharyngeal
Container	Sterile
Collection Instructions	The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma pneumoniae DNA is unlikely. Specimen source is required. Submit only 1 of the following specimens: Specimen Type: Respiratory Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum Container/Tube: Sterile container Specimen Volume: 1 mL (0.5 mL min) Specimen Type: Swab Specimen: Throat nasal, or nasopharyngeal Container/Tube: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT , M5, M6, UTM, or ESwab Specimen Volume: Swab Collection Instructions: 1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells. 2. Place swab back into swab cylinder. Specimen Type: Fluid Sources: Pleural, pericardial, cerebrospinal Container/Tube: Sterile vial Specimen Volume: 0.5 mL Transport Temperature: Refrigerated
Transport Instructions	Refrigerated
Specimen Stability	Varies 7 days Refrigerated (preferred) 7 days Frozen
Methodology	Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Days Performed	TAT 5-6 Days
Performing Laboratory	Mayo Medical Laboratories
CPT	87581 LOINC Code 29257-3
PDM	1759238
Result Interpretation Reference Range: Negative Interpretation A positive result indicates the presence of Mycoplasmoides pneumoniae. A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below the limits of detection of the assay.
Forms