Diagnosing infections due to Mycoplasma (Mycoplasmoides) pneumoniae Assessing macrolide susceptibility
Specimen Type
Body Fluid, CSF, Swab, Respiratory, Nasal, Nasopharyngeal
Container
Sterile
Collection Instructions
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma pneumoniae DNA is unlikely.
Specimen Type: Swab Specimen: Throat nasal, or nasopharyngeal Container/Tube: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT , M5, M6, UTM, or ESwab Specimen Volume: Swab Collection Instructions: 1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells. 2. Place swab back into swab cylinder.
Specimen Type: Fluid Sources: Pleural, pericardial, cerebrospinal Container/Tube: Sterile vial Specimen Volume: 0.5 mL Transport Temperature: Refrigerated
Transport Instructions
Refrigerated
Specimen Stability
Varies 7 days Refrigerated (preferred) 7 days Frozen
Methodology
Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
