IgVH Mutation Analysis IgVH Mut

Synonyms

Allscripts (AEHR) Order Name

IGVH Mutation Analysis

Sunrise Clinical Manager (SCM) Order Name

IgVH Mutation Analysis (CLL Prognosis)

EPIC Order Name

IgVH Mutation Analysis

Clinical Info

This test determines the mutation status of IGHV gene in B lymphocytes, including those of CLL (chronic lymphocytic leukemia).
The IGHV gene mutation status is one of the discriminators of clinical outcome in patients with CLL. The mutational status of the
immunoglobulin genes expressed by CLL cells can be used to segregate patients into two subsets that have significantly different 
tendencies for disease progression. Patients with leukemic cells that express unmutated immunoglobulin heavy-chain variable region 
genes have a greater tendency for disease progression than those who have leukemic cells that express IGHV genes with less than 
98% nucleic acid homology with their germ-line counterparts.

Specimen Type

Blood, Bone Marrow

Specimen Volume

Whole Blood and bone Marrow volume: 1 mL (Min 0.5 mL)

Container

Peripheral blood or bone marrow

Collection Instructions

Container
Lavender-top (EDTA) tube, green-top (heparin) tube or yellow-top (ACD) tube                           

Transport Instructions

Room Temperature

Specimen Stability

Maintain specimen at room temperature; stable for three to five days.

Methodology

The immunoglobulin heavy-chain gene variable region (IGHV) somatic hypermutation analysis is performed using extracted patient RNA
as starting material. Subsequent amplification of the IgH gene is performed by polymerase chain reaction (PCR). The PCR products are 
isolated and sequenced. 

Days Performed

TAT:  7-9 Days

Performing Laboratories

Labcorp

CPT

81263

PDM

1659924

Result Interpretation

Forms