Synonyms
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- IGVH MUTATION ANALYSIS (CLL PROGNOSIS)
- LAB10976
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Cerner Name
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IgVH Mutation Analysis
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Clinical Info
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This test determines the mutation status of IGHV gene in B lymphocytes, including those of CLL (chronic lymphocytic leukemia).
The IGHV gene mutation status is one of the discriminators of clinical outcome in patients with CLL. The mutational status of the
immunoglobulin genes expressed by CLL cells can be used to segregate patients into two subsets that have significantly different
tendencies for disease progression. Patients with leukemic cells that express unmutated immunoglobulin heavy-chain variable region
genes have a greater tendency for disease progression than those who have leukemic cells that express IGHV genes with less than
98% nucleic acid homology with their germ-line counterparts.
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Specimen Sources
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Blood, Arterial
Blood, Capillary
Blood, Central Line
Blood, Venous
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Specimen Types
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Blood
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Specimen Volume
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Whole Blood and bone Marrow volume: 1 mL (Min 0.5 mL)
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Container
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Peripheral blood or bone marrow
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Collection Instructions
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Container
Lavender-top (EDTA) tube, green-top (heparin) tube or yellow-top (ACD) tube
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Transport Instructions
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Room Temperature
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Specimen Stability
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Maintain specimen at room temperature; stable for three to five days.
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Methodology
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The immunoglobulin heavy-chain gene variable region (IGHV) somatic hypermutation analysis is performed using extracted patient RNA
as starting material. Subsequent amplification of the IgH gene is performed by polymerase chain reaction (PCR). The PCR products are
isolated and sequenced.
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Days Performed
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TAT: 7-9 Days
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Performing Laboratories
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Labcorp
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CPT
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81263
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PDM
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1659924
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Results
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| Component Name |
Base Name |
Common Name |
External Name |
| INTERPRETATION |
INTERP |
INTERPRETATION |
|
| IGVH MUTATION |
IGVHMUT |
IGVH MUTATION |
IGVH Mutation |
| IGVH FAMILY |
IGVHFAM |
IGVH FAMILY |
IgVH Family |
| MUTATION RATE |
MUTRATE |
MUTATION RATE |
Mutation Rate |
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Result Interpretation
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Forms
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