PML/RARA Quantitative, PCR PML/RARA

Synonyms

Allscripts (AEHR) Order Name

PML/RARA Quantitative, PCR

Sunrise Clinical Manager (SCM) Order Name

Promyelocytic Leukemia/Retinoic Acid Receptor A

Clinical Info

Diagnosis of acute promyelocytic leukemia (APL)

Detection of residual or recurrent APL

Monitoring the level of promyelocytic leukemia/retinoic acid receptor alpha (PML/RARA) in APL patients

Specimen Type

Blood, Bone Marrow

Container

Lavender Top Tube

Collection Instructions

Submit only 1 of the following specimens:

Specimen Type: Whole blood
Container/Tube:
Preferred: 2 EDTA (lavender top)
Acceptable: ACD (yellow top)
Specimen Volume: 10 mL (8 mL min)
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
3. Label specimen as blood.

Specimen Type: Bone marrow
Container/Tube:
Preferred: EDTA (lavender top)
Acceptable: ACD (yellow top)
Specimen Volume: 4 mL (2 mL min)
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send specimen in original tube.
3. Label specimen as bone marrow.
Stability: 5 days Refrigerated (preferred)
72 hours Ambient

Transport Instructions

Specimen Stability

5 days Refrigerated (preferred)
72 hours Ambient

Methodology

Quantitative, Real-Time Polymerase Chain Reaction (PCR)

Days Performed

Monday through Friday
TAT: 5-10 Days

Performing Laboratory

Mayo Medical Laboratories

CPT

81315-PML/RARalpha (t(15;17)), (PML-RARA regulated adaptor
molecule 1) (eg promyelocytic leukemia) translocation analysis; all
breakpoints (eg, intron 3, intron 6 and variable in exon 6),
qualitative or quantitative

PDM

1553720

Result Interpretation

An interpretive report will be provided.

 

If positive, a value representing a ratio of PML-RARA fusion transcript to the
control gene ABL1
expressed as a percentage will be reported.

Forms

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