Screening for mast cell activation disorders including systemic mastocytosis using 24-hour urine specimens 2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is elevated in the urine of patients with systemic mastocytosis (SM). This test should be used as a screening test for SM. When 2,3 BPG is used in combination with urinary leukotriene E4 and N-methyl histamine, the sensitivity for SM detection increases to 90%.
Specimen Sources
Urine, CatheterUrine, Clean Catch
Specimen Types
Urine
Container
24hr Ur No Preserv
Collection Instructions
Container/Tube: 24 Hr Urine container. No preservative Specimen : 5 mL (4 mL min) Transport Temperature: Refrigerated Collection Instructions: 1. Collect urine for 24 hours. 2. Refrigerate specimen during collection, and send specimen refrigerated. Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens in Special Instructions for multiple collections. Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAID) may have decreased concentrations of prostaglandin F2 alpha. If possible, discontinue for 2 weeks or 72 hours, respectively, prior to collecting a specimen.
Specimen Volume
5 mL (4 mL min)
Transport Instructions
Refrigerated
Specimen Stability
Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Enzymatic Colorimetric Assay
