Clinical Significance: Prostaglandins are fatty acids derived from arachidonic acid metabolism. They are closely related to the Thromboxanes and Leukotrienes. Prostaglandin D2 is derived mainly from Prostaglandin H2, and is metabolized to 13,14-dihydro-15-keto Prostaglandin D2. Prostaglandin D2 is excreted directly into the urine. The sites of highest Prostaglandin D2 activity are the brain, spinal cord, intestines, and stomach.
Prostaglandin D2 is the major Prostaglandin produced by uterine tissue. Prostaglandin D2 is a potent bronchoconstrictor, neuromodulator, and anti-thrombin agent. It also stimulates the secretion of Pancreatic Glucagon. Prostaglandin D2 has been found to have an anti-metastatic effect on many malignant tumor cells. Prostaglandin D2 production and circulating levels are drastically suppressed by aspirin and indomethacin.
Specimen Sources
Urine, Clean Catch
Specimen Types
Urine
Specimen Volume
5-10 mL Urine (Min Volume: 1 mL)
Container
Transfer 5 – 10 ml urine to urine transport container and freeze immediately.
Collection Instructions
24 hours urine collection. Refrigerate during collection and mix well. Transfer 5 – 10 ml urine to urine transport container and freeze immediately.
Minimum sample volume: 1 ml
Patient Preparation: Patient should not be on aspirin, indomethacin, or anti-inflammatory medications, if possible, for at least 48 hours prior to collection of specimen.
Transport Instructions
FROZEN Temp
Specimen Stability
Rejection criteria include: room temperature, thawed, or refrigerated specimens.
Methodology
Urine Prostaglandin D2 is measured by direct ELISA.
