Cytochrome P450 2C19 (CYP2C19) is a drug-metabolizing enzyme involved in the metabolism of several clinically important drugs including the platelet aggregation inhibitor clopidogrel, the tricyclic antidepressants amitriptyline and nortriptyline, the selective serotonin reuptake inhibitors citalopram and sertraline, and some proton pump inhibitors including omeprazole and pantoprazole.
Specimen Sources
Blood, ArterialBlood, CapillaryBlood, Central LineBlood, VenousOral Cavity
Specimen Types
Blood
Container
Lavender Top Tube
Collection Instructions
Container/Tube: Lavender top or Yellow ACD Tube or Buccal Swab Specimen; 3 mL Whole Blood (1.0 mL min) 4 Buccal Swabs (2 Swabs min) Causes for Rejection: Quantity not sufficient for analysis; buccal swab; buccal swabs without outer collection envelope; severely damaged buccal swab envelope; buccal swab envelope received open; frozen glass tube
Transport Instructions
Room Temperature
Specimen Stability
Room Temperature Whole Blood: 28 days Swabs: 2 Months Refrigerated Whole Blood: 28 days Swabs: Unstable Frozen Whole Blood 2 Years Swab Unacceptable
Methodology
DNA analysis is performed by allele-specific real-time polymerase chain reactions (RT-PCR) to detect single-nucleotide polymorphisms (SNPs) within the CYP2C19 gene and to assign variant CYP2C19 *2, *3, *4, *5, *6, *7, *8, *9, *10, *17 and *35 alleles. *1 denotes detection of the reference (wild-type) sequence at the assessed alleles. No other variants in this gene are detected by this assay.
Days Performed
TAT: 8-11 Days
Performing Laboratory
Monogram Biosciences Inc 345 Oyster Point Blvd, S San Francisco, CA
