Bacterial Broad Range 16S Amplicon Molecular Detection and Sequencing

Synonyms

• BRBPS
• BROAD RANGE BACTERIA PCR+SEQUENCING
• LAB11852

Cerner Name

Broad Range Bacteria PCR+Sequencing

Clinical Info

Detecting and identifying bacteria (including mycobacteria) from normally sterile sources, including synovial fluid; body fluids such as pleural, peritoneal, and pericardial fluids, cerebrospinal fluid; and both fresh and formalin-fixed paraffin-embedded tissues

This test is not recommended as a test of cure because nucleic acids may persist for long periods of time after successful treatment.
This test is used for detection and identification of bacteria (including mycobacteria) in normally sterile specimens.

Specimen Sources

Adenoids Amniotic Sac Ankle, Left Ankle, Right Aortic Valve Arm, Left Arm, Right Back, Lower Back, Upper Bile Duct Bladder Brain Breast, Left Breast, Right Buttock, Left Buttock, Right Cervix Cheek, Left Cheek, Right Chest Chin Colon Decubitus ulcers Duodenum Ear, Left Ear, Right Elbow, Left Elbow, Right Eye, Left Eye, Right Fibroids Finger, Left Finger, Right Foot, Left Foot, Right Forehead Frontal sinus Gallbladder Gastric Groin Hand, Left Hand, Right Hip, Right Jaw, Left Jaw, Right Kidney, Left Kidney, Right Knee, Left Knee, Right Labia Larynx Leg, Left Leg, Right Lip, Lower Lip, Upper Liver Lung, Left Lower Lobe Lung, Left Upper Lobe Lung, Right Lower Lobe Lung, Right Middle Lobe Lung, Right Upper Lobe Lymph Node Mitral Valve Nares Nasopharynx Navel Neck Oropharynx Ovarian Cyst Ovary Pancreas Paranasal Pelvis Penis Pericardium Peritoneal Pharynx Placenta Pleura, Left Pleura, Right Prostate Prosthetic valve Rectum Scalp Scrotum Shoulder, Left Shoulder, Right Sinus, Nasal Skull Spleen Thyroid Toe, Left Toe, Right Tongue Tonsil Trachea Tricuspid valve Urethra Vertebra Vulva Wrist, Left Wrist, Right Show more...

Specimen Types

Tissue

Specimen Volume

Fresh tissue is preferred over formalin-fixed, paraffin-embedded tissue.

Container

Sterile

Collection Instructions

Testing Algorithm:
If polymerase chain reaction (PCR) testing is negative, no sequencing is performed, and the test is resulted as negative.
If PCR testing is positive, sequencing is performed. Strong positive results are first submitted to Sanger sequencing, which can yield results in as few as 4 days. Weak positive results, or Sanger sequencing results that are mixed, are submitted to next-generation sequencing (ie, targeted metagenomics testing).

Specimen: Fresh tissue is preferred over formalin-fixed, paraffin-embedded tissue.
Submit only 1 of the following specimens:

Preferred Specimen Type:
Specimen Type: Fresh tissue or biopsy
Sources: Normally sterile tissue such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung
Container/Tube: Sterile container
Specimen Volume: Entire collection or 5 mm(3)-approximately the size of a pencil eraser
Collection Instructions:
1. Collect fresh tissue specimen.
2. Submit tissue only, do not add fluid to tissue.
3. Freeze specimen.
Specimen Stability Information: Frozen <14 days (preferred)/Refrigerated <14 days

Alternate Specimen Type:
Preferred: Paraffin-embedded tissue block:
Supplies: Tissue Block Container
Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block
Sources: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.
Specimen Stability Information: Ambient (preferred)/Refrigerated

Acceptable: Paraffin-embedded tissue block:
Specimen Type: Section (scrolls) of FFPE tissue block
Sources: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung
Container/Tube: Sterile container for each individual cut section (scroll)
Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.
Specimen Stability Information: Ambient (preferred)/Refrigerated

Specimen Type: Fluid
Sources: Normally sterile body fluids such as cerebrospinal, vitreous humor, pleural, abdominal, peritoneal, ascites, pericardial, pelvic
Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions:
1. Collect fresh fluid specimen.
2. Freeze specimen.
Specimen Stability Information: Frozen <14 days(preferred)/Refrigerated <14 days

Specimen Type: Synovial fluid
Container/Tube:
Preferred: Red top or sterile container
Acceptable: Lavender top (EDTA), pink top (EDTA), royal blue top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 1 mL
Collection Instructions: Send specimen in original tube (preferred).
Specimen Stability Information: Frozen <14 days (preferred)/ Refrigerated <14 days

Transport Instructions

Specimen Stability

Methodology

16S Ribosomal RNA Gene Polymerase Chain Reaction (PCR) followed by Sequencing

Days Performed

TAT: 14-29 Days

Performing Laboratories

Mayo Medical Laboratories

CPT

87801
87798-Bacterial Ident by Sequencing (if appropriate)
87798-Specimen Identification by PCR (if appropriate)
87798-Ident by Next Generation Sequencing (if appropriate)
87483-Meningitis Encephalitis Panel, PCR (if appropriate)
LOINC Code: 76575-0

PDM

2114059

Results

Result Interpretation

Forms