Broad Range Bacteria PCR & Sequencing

Broad Range Bacteria PCR+Sequencing

Detecting and identifying bacteria (including mycobacteria) from normally sterile sources, including synovial fluid; body fluids such as pleural, peritoneal, and pericardial fluids, cerebrospinal fluid; and both fresh and formalin-fixed paraffin-embedded tissues This test is not recommended as a test of cure because nucleic acids may persist for long periods of time after successful treatment. This test is used for detection and identification of bacteria (including mycobacteria) in normally sterile specimens.

Body Fluid, Tissue, Other

Sterile Cntr

Testing Algorithm: If polymerase chain reaction (PCR) testing is negative, no sequencing is performed, and the test is resulted as negative. If PCR testing is positive, sequencing is performed. Strong positive results are first submitted to Sanger sequencing, which can yield results in as few as 4 days. Weak positive results, or Sanger sequencing results that are mixed, are submitted to next-generation sequencing (ie, targeted metagenomics testing). Specimen: Fresh tissue is preferred over formalin-fixed, paraffin-embedded tissue. Submit only 1 of the following specimens: Preferred Specimen Type: Specimen Type: Fresh tissue or biopsy Sources: Normally sterile tissue such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung Container/Tube: Sterile container Specimen Volume: Entire collection or 5 mm(3)-approximately the size of a pencil eraser Collection Instructions: 1. Collect fresh tissue specimen. 2. Submit tissue only, do not add fluid to tissue. 3. Freeze specimen. Specimen Stability Information: Frozen <14 days (preferred)/Refrigerated <14 days Alternate Specimen Type: Preferred: Paraffin-embedded tissue block: Supplies: Tissue Block Container Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block Sources: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung Container/Tube: Tissue block Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned. Specimen Stability Information: Ambient (preferred)/Refrigerated Acceptable: Paraffin-embedded tissue block: Specimen Type: Section (scrolls) of FFPE tissue block Sources: Normally sterile or deep tissues such as bone, lymph node, joint, heart valve, brain, viscera, organ, lung Container/Tube: Sterile container for each individual cut section (scroll) Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission. Specimen Stability Information: Ambient (preferred)/Refrigerated Specimen Type: Fluid Sources: Normally sterile body fluids such as cerebrospinal, vitreous humor, pleural, abdominal, peritoneal, ascites, pericardial, pelvic Container/Tube: Screw-capped, sterile container Specimen Volume: 1 mL Collection Instructions: 1. Collect fresh fluid specimen. 2. Freeze specimen. Specimen Stability Information: Frozen <14 days(preferred)/Refrigerated <14 days Specimen Type: Synovial fluid Container/Tube: Preferred: Red top or sterile container Acceptable: Lavender top (EDTA), pink top (EDTA), royal blue top (EDTA), or sterile vial containing EDTA-derived aliquot Specimen Volume: 1 mL Collection Instructions: Send specimen in original tube (preferred). Specimen Stability Information: Frozen <14 days (preferred)/ Refrigerated <14 days

16S Ribosomal RNA Gene Polymerase Chain Reaction (PCR) followed by Sequencing

TAT: 14-29 Days

Mayo Medical Laboratories

87801 87798-Bacterial Ident by Sequencing (if appropriate) 87798-Specimen Identification by PCR (if appropriate) 87798-Ident by Next Generation Sequencing (if appropriate) 87483-Meningitis Encephalitis Panel, PCR (if appropriate) LOINC Code: 76575-0

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