JAK2 V617F Mutation, Qualitative with Reflex to Exon12

JAK2 with Reflex Panel

Aiding in the distinction between the myeloproliferative neoplasm polycythemia vera and other secondary erythrocytosis.

Blood, Bone Marrow

Lavender Top Tube

Container/Tube: 2 Lavender Top (EDTA) Tube Specimen:10 ml whole blood (8 mL min) 4 mL Bone Marrow (2 mL min) Transport Temperature: Refrigerated whole blood or Bone Marrow

Refrigerated whole blood

5 Days Room Temperature 5 days Refrigerated Frozen: Unacceptable

Taqman® real time PCR amplification/detection Sanger sequencing TESTING ALGORITHM Both DNA and RNA are extracted. The algorithm starts with a highly sensitive DNA-based JAK2 V617F test by allele specific polymerase chain reaction. If the JAK2 V617F result is negative or very low positive (0.06%-0.6%), JAK2 exon 12-15 Sanger sequencing will be performed on the stored RNA sample. If a JAK2 V617F mutation (>0.6%) is detected, no further testing will be performed. The Sanger sequencing covers JAK2 exons 12 through the first 90% of exon 15, which spans the region containing essentially all mutations reported in myeloproliferative neoplasms.

TAT: 8 - 13 Days

Mayo Medical Laboratories

81270   LOINC Code: 43399-5

1659066