Testosterone, Free MS/EQ Dialysis TESTMS

Synonyms
Allscripts (AEHR) Order Name	Testosterone, Free MS/EQ Dialysis
Sunrise Clinical Manager (SCM) Order Name	Testosterone, Free MS/EQ Dialysis
EPIC Order Name	Free Testosterone by Mass Spectrometry/Equilibrium Dialysis
Clinical Info	Measurement of total and free testosterone is key in diagnosis of androgen excess or deficiency. Free testosterone is best measured by equilibrium dialysis, while total testosterone is measured by HPLC with tandem mass spectrometry.1-3 Free testosterone is estimated in this profile using these recommended techniques. Tritiated testosterone is added to the sample and allowed to dialyze across a membrane. Once equilibrium has been achieved, the solutions on both sides of the membrane are sampled to determine the percent free testosterone. Total testosterone is measured by HPLC with tandem mass spectrometry. Serum free testosterone is then calculated by multiplying the percent free testosterone by the total testosterone concentration with unit correction. Limitations This method is recommended for testing women, children, and hypogonadal men.
Specimen Type
Container	Gold Top Tube
Collection Instructions	Container/Tube: Gold Top Tube Specimen: 2 mL serum ( 1 mL min) Transport Temperature Refrigerated Collection Serum must be separated from cells within 45 minutes of venipuncture.
Transport Instructions
Specimen Stability	6 Days Room Temperature 7 Days Refrigerated 2.6 Years Frozen
Methodology	Testosterone: high-pressure liquid chromatography (HPLC)/tandem mass spectrometry; free testosterone: equilibrium dialysis
Days Performed	TAT: 6 - 10 Days
Performing Laboratory	LabCorp- Endocrine Sciences
CPT	84402 84403 LOINC Code: 2991-8
PDM	225709
Result Interpretation See Report
Forms