C4B Binding Protein C4BPROTEIN

Synonyms

Allscripts (AEHR) Order Name

C4B Binding Protein

Sunrise Clinical Manager (SCM) Order Name

C4B Binding Protein

EPIC Order Name

C4B Binding Protein

Clinical Info

Confirmation and characterization of protein S (PS) deficiency: C4b-binding protein elevates as an acute phase protein that may lead to enhanced protein S binding and decreased free protein S levels.

Specimen Type

Blood

Specimen Volume

1 mL plasma (0.5mL min)

Container

ESR

Collection Instructions

Container/Tube:  Light blue-top (3.2% sodium citrate) tube
Specimen:   1 mL plasma (0.5mL min)
 
Collection: Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used. When non citrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube.

Transport Instructions

Frozen plasma

Specimen Stability

8 Hours Room Temperature
14 Days Frozen

Methodology

Enzyme-linked immunosorbent assay (ELISA)

Days Performed

TAT: 5-9 Days

Performing Laboratory

Esoterix Endocrinology

CPT

83520

PDM

5601085

Result Interpretation

Forms