Synonyms
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Allscripts (AEHR) Order Name
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Culture - Acid Fast - Tissue w/Smear
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Sunrise Clinical Manager (SCM) Order Name
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Culture - Acid Fast - Tissue w/Smear
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EPIC Order Name
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Tissue Mycobacterial (AFB) Smear and Culture
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Clinical Info
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Mycobacteria, particularly Mycobacterium tuberculosis,
pose a significant global public health challenge, with early diagnosis being
crucial for preventing transmission and initiating prompt treatment.
Additionally, approximately 200 known species of nontuberculous mycobacteria
(NTM), commonly found in the environment (soil and water), are emerging as
opportunistic human pathogens, with a rising prevalence of pulmonary NTM
infections.
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Specimen Type
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Specimen Volume
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5-10 mm
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Container
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Sterile
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Collection Instructions
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Submit only 1 of the following specimens:
Fresh Tissue
1. Obtain fresh tissue (size 5-10mm3 in size)and place specimen in a screw-capped, sterile container.
2. Label container with patient’s name (first and last), date and actual time of collection, and type of specimen.
3. Send specimen refrigerated.
4. Maintain sterility and forward promptly.
Note: Specimen source is required on request form for processing.
* Rejection Criteria: Quantity not sufficient, specimen leaking, unlabeled or mislabeled specimen, specimen without a minimum of 2 patient identifiers, specimen received after prolonged delay (more than 7 days), specimen in fixative.
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Transport Instructions
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Refrigerated
(2°-8°C), transport to the laboratory as soon as possible.
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Specimen Stability
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Up to 3 days at 2°-8°C (refrigerated)
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Methodology
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The specimen is processed and
an acid fast smear is performed to
detect acid-fast bacilli. . All specimens are cultured in broth and
solid media for up to 6 weeks for the detection and identification of M.
tuberculosis complex and nontuberculous mycobacteria.
M. tuberculosis complex isolates are referred to the department of
health for further speciation and susceptibility testing.
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Days Performed
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Monday through Sunday
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Performing Laboratories
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Northwell Health Laboratories -Little Neck Parkway
If culture is positive, additional charge(s)/CPT code(s) may apply for identification
and/or antibiotic susceptibilities performed when appropriate.
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CPT
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87116 - culture
87206 - smear
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PDM
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6201260
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Result Interpretation
Acid-fast smear = No acid-fast bacilli seen by fluorochrome stain.
Culture = No acid-fast bacilli isolated after 6 weeks
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Forms
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