Herpes Viral Culture Rlx to Typing V VCH

Synonyms

Allscripts (AEHR) Order Name

HSV Culture and Typing

Sunrise Clinical Manager (SCM) Order Name

Culture - HSV/Type

EPIC Order Name

Herpes Simplex Virus (HSV) Culture and Typing

Clinical Info

HSV culture is useful in verifying HSV is present within the vesicles.

Specimen Type

Body Fluid, CSF, Urine, Swab, Tissue, Vaginal

Specimen Volume

Submit only 1 of the following specimens:

Container

Viral Trn

Collection Instructions

Container/Tube: VTM media
Body fluids and tissue samples may be submitted in viral transport media or in a sterile leakproof container.
Specimen:  Submit only 1 of the following specimens:
 
Acceptable Specimens:
Vesicular fluid, ulcerated lesions, pharyngeal and throat swabs, urine, cerebrospinal fluid (CSF), autopsy and biopsy material, eye exudates, vaginal swabs.
 
Transport Temperature:  Refrigerated
Stability:  VTM/UTM/M4 transport medium
                       Refrigerated: 7 Days
                
Collection Instructions:
Specimen is best collected within the first three days after appearance of lesion but no more than seven days. After collection, remove cap from the transport media tube and insert swab aseptically into transport tube and break swab shaft. Replace cap so that swab will not interfere with closure and allow tube to leak. Label tube with patient identification and transport to the laboratory at 4°C.
Causes for Rejection: Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport conditions; specimen stored or transported greater than seven days refrigerated; specimen stored or transported at room temperature; wooden shaft swab in transport device; unlabeled specimen or name discrepancy between specimen and test request label.

Transport Instructions

Refrigerated

Specimen Stability

VTM/UTM/M4 transport medium
                       Refrigerated: 7 Days
                       

Methodology

Enzyme-linked virus-inducible system (ELVIS®). ELVIS® HSV was developed using a transgenic baby hamster kidney-21 cell line. In this cell line, HSV infection causes the expression of a beta-galactosidase “reporter gene”, and it is the expression of this gene that can be used to monitor infection by HSV. Cultures positive by ELVIS® are confirmed by immunofluorescent staining.

Days Performed

3 - 5 day TAT

Performing Laboratories

LabCorp

CPT

87255

PDM

6400115

Result Interpretation

Forms