Rapid AML Panel RapidAML

Synonyms

OnkoAML

Allscripts (AEHR) Order Name

Rapid AML Panel

Sunrise Clinical Manager (SCM) Order Name

Rapid AML Panel

EPIC Order Name

Clinical Info

Use
Mutations in the FLT3 gene are common mutations in acute myeloid leukemia (AML). This assay detects internal tandem duplication (ITD) mutations and mutations in the tyrosine kinase domain (TKD) of FLT3. Presence of these mutations in AML provide prognostic information and can aid in the 
determination of therapeutic regimen.

Specimen Type

Blood, Bone Marrow

Specimen Volume

3-5 mL whole blood or 1-2 mL bone marrow
Minimum Volume:   3 mL whole blood or 1 mL bone marrow

Container

 Lavender-top (EDTA) tube OR green-top (sodium heparin)
tube

Collection Instructions

Lavender-top (EDTA) tube OR green-top (sodium heparin)
tube whole blood or bone marrow
Causes for Rejection
Specimen does not meet collection criteria; frozen
whole blood, marrow or cell pellet; leaking tube; clotted blood or marrow;
grossly hemolyzed specimen or otherwise visibly degraded; contamination by
another specimen; specimens containing suspicious foreign material

Transport Instructions

Room temperature

Specimen Stability

Specimen should
arrive in the laboratory within 48 hours of collection.

Methodology

 FLT3: Polymerase chain reaction (PCR); restriction
enzyme digest; capillary electrophoresis
NPM1: Polymerase chain reaction (PCR); capillary
electrophoresis
IDH1/2 SNaPshot: Multiplex PCR (primer extension-based
method)

Days Performed

TAT:4-6 Days

Performing Laboratories

• NWH CL Send Outs-Labcorp

CPT

81120,81121,81245,81246,81310

PDM

255569

Result Interpretation

Forms