Synonyms
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Trichomonas, Trichomoniasis, Vaginitis, Vaginosis, STI
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Allscripts (AEHR) Order Name
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Trichomonas Vaginalis
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Sunrise Clinical Manager (SCM) Order Name
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Trichomonas Vaginalis Amplification
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EPIC Order Name
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Trichomonas vaginalis Molecular Detection
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Clinical Info
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For molecular detection of Trichomonas vaginalis from urogenital sources.
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Specimen Type
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Urine, Swab, Cervical, Vaginal
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Specimen Volume
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2mL of urine in Hologic Aptima tube. Endocervical swab or vaginal swab in Hologic Aptima tube, or ThinPrep vial
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Container
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Aptima Multitest Swab Specimen Collection Kit
Aptima Urine Collection Kit for Male and Female Urine Specimens
Aptima Unisex Swab Specimen Collection Kit
ThinPrep vial (PreservCyt)
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Collection Instructions
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Urogenital Swab Specimens
a. Endocervical swab specimens
1. Remove excess mucus from the cervical os and surrounding mucosa using the cleaning swab (white
shaft swab in the package with red printing). Discard this swab.
Note: To remove excess mucus from the cervical os, a large-tipped swab (not provided) may be used.
2. Insert the specimen collection swab (blue shaft swab in the package with the green printing) into the
endocervical canal.
3. Gently rotate the swab clockwise for 10 to 30 seconds in the endocervical canal to ensure adequate
sampling.
4. Withdraw the swab carefully; avoid any contact with the vaginal mucosa.
5. Remove the cap from the swab specimen transport tube and immediately place the specimen collection
swab into the transport tube.
6. Carefully break the swab shaft against the side of the tube at the scoreline and discard the top portion of
the swab shaft; use care to avoid splashing of contents.
7. Re-cap the swab specimen transport container tightly.
2. Urine Specimens
The patient should not have urinated for at least 1 hour prior to specimen collection.
2. Direct patient to provide a first-catch urine (approximately 20 to 30 mL of the initial urine stream) into a
urine collection cup free of any preservatives. Collection of larger volumes of urine may result in target
dilution that may reduce test sensitivity. Female patients should not cleanse the labial area prior to
providing the specimen.
3. Remove the cap and transfer 2 mL of urine into the urine specimen transport tube using the disposable
pipette provided. The correct volume of urine has been added when the fluid level is between the black fill
lines on the urine specimen transport tube label.
4. Re-cap the urine specimen transport tube tightly. This is now known as the processed urine specimen.
3. PreservCyt Solution Liquid Pap Specimens
a. Transport and store the PreservCyt solution specimen at 2°C to 30°C for up to 30 days.
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Transport Instructions
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Aptima Multitest Swab or Unisex Swab Specimen: After collection, transport and store the swab in the swab specimen transport tube at 2°C to 30°C until tested. Specimens must be assayed with the Aptima assays within 60 days of collection.
Urine: Transfer to Aptima tube within 24 hours. Transport the processed urine specimens in the Aptima urine specimen transport tube at 2°C to
30°C and store at 2°C to 30°C until tested. Processed urine specimens should be assayed with the Aptima assay
within 30 days of collection
PreservCyt solution specimen at 2°C to 30°C for up to 30 days.
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Specimen Stability
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Aptima Multitest Swab or Unisex Swab Specimen: After collection, transport and store the swab in the swab specimen transport tube at 2°C to 30°C until tested. Specimens must be assayed with the Aptima assays within 60 days of collection.
Urine: Transfer to Aptima tube within 24 hours. Transport the processed urine specimens in the Aptima urine specimen transport tube at 2°C to 30°C and store at 2°C to 30°C until tested. Processed urine specimens should be assayed with the Aptima assay within 30 days of collection
PreservCyt solution specimen at 2°C to 30°C for up to 30 days.
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Methodology
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Nucleic Acid Amplification
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Days Performed
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TAT: 2 - 4 Days
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Performing Laboratories
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- Core Lab Laboratory
- Northwell Health-CFAM
- SIUH Pouch Terminal Laboratory
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CPT
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87661
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PDM
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6350820
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Result Interpretation
T. vaginalis rRNA: Not detected
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Forms
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