JAK2 Exon 12, 13, 14, and 15 Mutation JAK2EXON

Synonyms

Allscripts (AEHR) Order Name

JAK2 Exons 12-13

Sunrise Clinical Manager (SCM) Order Name

JAK2 Exon 12, 13, 14, and 15 Mutation

EPIC Order Name

JAK2 Exon 12, 13, 14, and 15 Mutation

Clinical Info

Second-order testing to aid in the distinction between a reactive cytosis and a myeloproliferative neoplasm, particularly when a diagnosis of
 polycythemia is being considered, using blood specimens

Specimen Type

Blood

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender EDTA tube
Specimen: 10 mL whole blood (5 mL min)
Transport Temperature: Refrigerated.

Note: Ship immediately to maintain sample stability.

Transport Instructions

Refrigerated.

Specimen Stability

5 days Room temperature
5 days Refrigerated
Unacceptable Frozen

Note: Ship immediately to maintain sample stability.

Methodology

Sanger Sequencing
Testing Algorithm
his is a second-order test that should be used when the test for the JAK2B / JAK2 V617F Mutation Detection, Blood test is negative.
The sensitivity of this assay is much less than that of the JAK2B test. This is because the sequencing technique is required to evaluate
for many potential mutations. The sensitive JAK2B test should always be performed first, as the JAK2 mutation burden may be very
low in some specimens. If the JAK2B test is negative, then this assay should be performed for detection of non-V617F JAK2 mutations.

Days Performed

Monday - Friday

Performing Laboratory

Mayo Medical Laboratories

CPT

0027U

PDM

5907501

Result Interpretation

Negative for JAK2 mutation

Forms

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