HLX IGR by PCR Case MG IGR

Synonyms

Allscripts (AEHR) Order Name

Not Orderable

Sunrise Clinical Manager (SCM) Order Name

Not Orderable

Clinical Info

Leukemias and lymphomas are characterized by the presence of clonal B-or T-cell populations. Rearrangement of the antigen receptor genes occur during the ontogeny of B and T cell lymphocytes. The rearrangements are unique in length and sequence for each cell. Immunoglobulin heavy chain (IGH) and Kappa Light chain (IGK) gene rearrangement assays are useful for the identification of clonal B-cell 
populations. Immunoglobulins are composed of heavy (IGH) and light (IGK and IGL) chains and are synthesized in B-cells by rearrangement of genes containing Variable, Diversity, Constant and Joining 
regions. Each one is the product of rearrangement of the Variable, Diversity, Constant and Joining regions of the respective genes. Each B- and T-cell has a single productive V-J rearrangement that is unique in both length and sequence. 

Specimen Type

Blood, Body Fluid, Bone Marrow, Tissue, Other

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender Top Tube or Sterile Container
Specimen: 1-3 mL of Peripheral Blood or Bone Marrow Aspirate collected in lavender top (EDTA) tube, transport at room temperature or 4°C.
Pleural Fluid (thoracentesis fluid) and fine needle aspirate (FNA) specimens collected in a sterile tube, transport at 4°C, submit at least 0.5 x 10^7 cells.
Formalin fixed paraffin embedded(FFPE) tissue, specimens should be representative of the tumor (contain neoplastic cells) as determined per pathologist.
5-10 Sections, 10 um thick. FFPE tissue can be stored indefinitely at room temperature.
Transport Temperature: Blood or Bone marrow: Room temperature or Refrigerated. Do Not Freeze,       FFPE: Room temperature, FNA and Pleural Fluid: Refrigerated

Transport Instructions

room temperature or 4°C

Specimen Stability

Blood or Bone marrow; 7 Days Refrigerated. Do Not Freeze
FFPE Indefinitely at room temperature
FNA and Pleural Fluid 7 Days Refrigerated

Methodology

Multiplex Polymerase Chain Reaction (PCR) Amplification and Capillary Electrophoresis

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

81261
81264

PDM

5160170

Result Interpretation

An interpretative report will be provided.

Forms

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