HLX BCR-ABL by RT-PCR CASE HLX BCR-ABL by RT-PCR CASE

Synonyms

Allscripts (AEHR) Order Name

BCR/ABL Quantitative

Sunrise Clinical Manager (SCM) Order Name

Not Orderable

Clinical Info

CML is a myeloproliferative disorder characterized by the presence of the Philadelphia (Ph) chromosome. This chromosome is the product of t (9; 22), a reciprocal translocation between the long arms of chromosomes 9 and 22. The corresponding fusion gene, BCR-ABL1, is transcribed into an 8.5 KB mRNA, with 2 junction variants b2a2 (40% of cases) and b3a2 (55% of cases).  The b2a3 and b3a3 transcripts represent less than 5% of cases.  The fusion gene encodes for a chimeric protein, p210, with elevated tyrosine kinase activity.

Specimen Type

Blood, Bone Marrow

Container

Lavender Top Tube

Collection Instructions

Peripheral Blood or Bone Marrow
Container/Tube: Lavender-top (EDTA) tube(s)
Specimen: 1-3 ml collected in EDTA (lavender top tubes). 
Transport Temperature: Refrigerated
Collection Instructions: 
Specimens should be stored in the refrigerator at 4ºC. Specimens should be processed within 3 days. 

Transport Instructions

Refrigerated

Specimen Stability

3 days Refrigerated

Methodology

Real-Time Polymerase Chain Reaction (PCR)

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

83891 - DNA extract-purify
83898 x 2 - amplification
83902 - reverse transcription

PDM

5911120

Result Interpretation

An interpretative report will be provided.

Forms

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