Not Orderable

Trichomonas vaginalis, Male (MTRNA)

Detection of Trichomonas vaginalis in urine and male patient specimens
Trichomonas vaginalis (TV) is a protozoan parasite that commonly infects the genital tract of men and women. It is now considered to be the most common curable sexually transmitted infection (STI) agent, with an estimated 3.7 million infected individuals in the United States.(1-4) Although up to 70% of infected individuals are asymptomatic, infections may be associated with vaginitis, urethritis, and cervicitis in women, and urethritis and prostatitis in men.(3) Patients that are infected with T vaginalis have an increased risk of acquiring other sexually transmitted infections such as HIV, while infections in pregnant women are associated with premature labor, low birth-weight offspring, premature rupture of membranes, and post-hysterectomy/post-abortion infection.(3)
 
Symptoms of T vaginalis overlap considerably with other sexually transmitted infections; therefore, laboratory diagnosis is required for definitive diagnosis. The most commonly used method for detection is microscopic examination of a wet-mount preparation of vaginal secretions. However, this method has only 35% to 80% sensitivity compared with culture.(5) Culture also suffers from relatively low sensitivity (38%-82%) when compared to molecular methods.(5) Culture is technically challenging and takes 5 to 7 days to complete. Molecular methods, such as the Aptima T vaginalis assay, offer high sensitivity and specificity for detection of trichomoniasis. The Aptima test utilizes target capture, transcription-mediated amplification (TMA), and hybridization protection assay (HPA) technologies for detection of T vaginalis ribosomal RNA (rRNA).

Specimen Type: Varies
Submit only 1 of the following specimens:
 
Specimen Type: Urine
Supplies: Aptima Urine Transport Tube (T582)
Container/Tube: Aptima Urine Specimen Transport Tube
Specimen Volume: 15 to 20 mL
Collection Instructions:
1. Patient should not have urinated for at least 1 hour prior to specimen collection.
2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.
3. Transfer 2 mL of urine into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.
 
Specimen Type: Urine (following prostatic massage)
Supplies: Aptima Urine Transport Tube (T582)
Container/Tube: Aptima Urine Specimen Transport Tube
Specimen Volume: 15 to 20 mL
Collection Instructions:
1. Patient should not have urinated for at least 1 hour prior to specimen collection.
2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded or submitted for other testing as applicable.
3. Patient then ceases voiding and a prostatic massage is performed by the urologist or other health care professional.
4. Collect post-massage urine into a sterile, plastic, preservative-free container.
5. Transfer 2 mL of post-massage urine specimen into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.
 
Specimen Type: Urethral
Supplies: Swab, Aptima Male/Female Collection (T583)
Container/Tube: Aptima Collection Unisex Swab
Specimen Volume: Swab
Collection Instructions:
1. Urethral specimens must be collected using an Aptima Collection Unisex Swab.
2. Patient should not have urinated for at least 1 hour prior to collection.
3. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.
4. Once inserted, rotate swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow swab to remain inserted for 2 to 3 seconds.
5. Place swab in the Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
6. Cap tube securely and label tube with patient's entire name and collection date and time.
Specimen Minimum Volume: See Specimen Required
Specimen Stability Information:

Transcription Mediated Amplification

Monday through Saturday

Mayo Clinic Laboratories in Rochester Please order in house TRICH AMP for this types of samples

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2059701