Synonyms
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- HEPARIN INDUCED PLATELET ANTIBODY WITH REFLEX
- HIPA
- HIPAX
- LAB4126
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Cerner Name
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Heparin Induced Platelet AB with Reflex
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Clinical Info
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Heparin‐induced thrombocytopenia (HIT) is a serious immune reaction to heparin exposure,
causing thrombocytopenia and increased clotting risk. Diagnosis involves 4T scoring, screening,
and confirmatory testing.
Heparin Induced Platelet AB with Reflex (HIPAX) is used as the initial screening test, which will now reflex to the confirmatory Serotonin Release Assay (SRA) performed by Versiti Diagnostic Laboratories based on the screening result. Heparin Induced Platelet Antibody ≥ 0.9 u/mL will reflex to Serotonin Release Assay
The Serotonin Releasing Assay will no longer be available to order separately. If the HIPAX test result is negative but clinically highly suspected for HIT, please contact the lab to request that the confirmatory Serotonin Release Assay (SRA) test be ordered and performed.
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Specimen Sources
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Blood, Arterial
Blood, Capillary
Blood, Central Line
Blood, Venous
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Specimen Types
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Blood
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Specimen Volume
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HIPA: 1.0 mL platelet poor citrated plasma
SRA: 5.0 mL serum
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Container
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1 Light Blue Citrated tube (HIPA) and 1 Red top tube (SRA)
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Collection Instructions
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HIPA:
Collection Instructions:
1. If the light blue top tube is the only tube to be drawn and will be collected using a butterfly set, a “discard” tube (either a plain[non-additive] or light blue) must be partially drawn just to completely fill the collection tubing with blood before collecting the light blue top for testing.
2. Allow blood to fill the tube completely to the line on the tube. The ratio of blood to anticoagulant is critical in coagulation testing and at least a 90% fill is required.
3. Immediately mix the specimen by gently inverting the tube 4-6 times to completely mix with the anticoagulant. Failure to perform this can result in a clotted sample, which is unacceptable for testing.
4. If centrifugation of the specimen cannot be performed immediately after collection, whole blood can be kept in the blue top tube at room temperature (18-24° C) for up to 4 hours.
5. If centrifugation is possible, and transport to the lab will not occur within 1 hour, spin down, remove the plasma to a clean plastic transport tube using a plastic transfer pipette, and spin the plasma again. Remove the plasma with a clean plastic transfer pipette to a fresh transport container and freeze this citrated platelet-poor plasma immediately. A double centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
6. Each coagulation assay requested should have its own vial of citrated plasma,1.0mL each.
SRA:
Collection Instructions:
1. Sample should be spun down and taken off the clot.
2. Sample must be received within 7 days of draw date if refrigerated.
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Transport Instructions
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Refrigerated
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Specimen Stability
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|
Methodology
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HIPA: Latex Immunoturbidimetric Assay
SRA: Serotonin Release Assay
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Days Performed
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Monday through Sunday
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Performing Laboratories
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Northwell Health Laboratories
Versiti Diagnostic Lab
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CPT
|
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PDM
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243283
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Results
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| Component Name |
Base Name |
Common Name |
External Name |
| HEPARIN-PF4 AB EIA |
HEPPF4ABEIA |
HEPARIN PF4 AB EIA |
Heparin-PF4 AB Result |
| HEPARIN-PF4 AB EIA |
HEPPF4ABEIA |
HEPARIN PF4 AB EIA |
Heparin-PF4 AB Result |
| HEPARIN-PF4 AB INTERP |
HEPABINTRP |
HEP AB INTERP |
Heparin-PF4 AB Interpretation |
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Result Interpretation
Heparin-PF4 AB Result: 0.0-0.9 u/mL
Interp: Negative
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Forms
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