Leptospira DNA Molecular Detection, Qualitative Build info

Synonyms

• LEPTOSPIRA DNA, QUAL PCR
• LEPTOPCR
• Leptospirosis
• LAB14307

Short Name

LEPTOSPIRA DNA, QUAL PCR

Procedure Master Number

LAB14307

Procedure ID

185299

Clinical Info

PCR can be used to rapidly diagnose Leptospirosis, an infection caused by a waterborne spirochete of the genus Leptospira.

Specimen Type

Blood, Urine

Container

Urine- Aptima Tube  
  or
Whole Blood- Lavender Top Tube

Collection Instructions

Container/Tube:  Urine  Aptima or sterile cup
                            Blood  or Urine is acceptable
Whole Blood- 1 mL Whole Blood  (0.8 mL min)
Urine -  5 mL Urine in Cup ( 1 mL min)-  MUST BE FROZEN
            or   2 mL urine in Aptima collection  
Urine Collection:  Aptima Collection:
2 mL of the urine specimen must be transferred into the APTIMA® Specimen Transport
within 24 hours of collection and before being assayed. The fluid (urine plus transport
media) level in the urine transport tube must fall within the clear pane of the tube label.

Transport Instructions

Whole Blood Refrigerated
Urine Aptima- Refrigerated
Urine in sterile cup- Frozen

Specimen Stability

Urine Aptima
  5 Days Room Temperature
  7 Days Refrigerated
 30 Days Frozen
Whole Blood
  2 Days Room Temperature
  7 Days Refrigerated
 30 Days Frozen
Urine not in Aptima- Unacceptable Room Temperature
                                 Unacceptable- Refrigerated
                                  30 Days Frozen
 

Methodology

Real-Time Polymerase Chain Reaction (RT-PCR)

Days Performed

TAT:  2-4 Days

Performing Laboratory

Quest Diagnostics Nichols Institute

CPT

87798
Loinc Code: 35491-0

PDM

245201

Results