Vitamin D, 25-Hydroxy

Synonyms

• 25 HYDROXYCALCIFEROL
• 25OHD
• D2
• D3
• LAB535
• VIT D
• VITAMIN D 25 HYDROXY

Cerner Name

Vitamin D 25 Hydroxy

Clinical Info

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy.

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous

Specimen Types

Blood

Specimen Volume

0.5 mL (minimum volume: 0.25 mL) of serum

Container

Gold Top Tube

Collection Instructions

Transport Instructions

Refrigerated

Specimen Stability

8 Hours Room Temperature
4 Days Refrigerated
24 weeks Frozen
Freeze only once

Methodology

Electrochemiluminescence Immunoassay (ECLIA)
This assay measures the total of D2(ergocalciferol) and D3(cholecalciferol).

Days Performed

Monday - Sunday, Continuously

Performing Laboratories

Northwell Health Laboratories

CPT

82306

PDM

5901360

Results

Result Interpretation

Forms

• Technical Memo Vitamin D 25-Hydroxy Test Update 03-03-2025