Evaluate porphyrias, including those involving deficiencies of enzymes that are needed for heme synthesis and chemical porphyrias.
Specimen Type
Urine
Container
24hr Ur No Light
Collection Instructions
Container/Tube: 24-Hour urine container.- Light Protected Specimen: 2 mL (1.5 mL min) . Collect with 5 grams of sodium carbonate or no preservative, if properly refrigerated and light protected Transport Temperature: Refrigerated urine- Indicate TV on container. Stability: Unstable Room Temperature Refrigerated: 7 days Frozen: 30 days Protect specimen from light during collection and transport
Collection Instruct patient to void at 8 AM (or 8 PM) and discard the specimen. Then collect all the urine, including the final specimen voided at the end of the 24-hour collection period (ie, 8 AM [or 8 PM] the following day). Specimen must be kept refrigerated
Transport Instructions
Refrigerated urine- Indicate TV on container.
Specimen Stability
Unstable Room Temperature Refrigerated: 7 days Frozen: 30 days Protect specimen from light during collection and transport
Collection Instruct patient to void at 8 AM (or 8 PM) and discard the specimen. Then collect all the
Methodology
High-pressure liquid chromatography (HPLC) with fluorometric detection
