Lymphocyte Subsets: B cell subsets (CD19/38/27/21/IgD/IgM)

Synonyms

  • B Cell Subset Analysis
  • BCELLSUB
  • LAB13509

Cerner Name

B Cell Subset Analysis

Clinical Info

***NOTE: This is a time sensitive assay and must be in the lab within 24 hours of draw***
Assess B cell subsets of immunodeficiencies. Support the diagnosis of common variable immune deficiency (CVID) and assist in predicting the clinical phenotype. Assess B cell reconstitution after bone marrow or hematopoietic stem cell transplantation.

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous

Specimen Types

Blood

Specimen Volume

4 mL whole Blood ( 2 mL min)

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender (EDTAS) Top Tube or Pink (K2EDTA) tube
Specimen; 4 mL whole blood ( 2 mL min)
Transport Temperature: Refrigerated
NOTE: This is a time sensitive assay and must be in the lab within 24 hours of draw

Transport Instructions

Specimen Stability

Unacceptable Room Temperature
48 Hours Refrigerated ( 24 to Northwell Labs)
Unacceptable Frozen

Methodology

Flow Cytometry

Days Performed

TAT: 2-4 Days

Performing Laboratories

ARUP Laboratories

CPT

86355
86356 x 6

PDM

235775

Results

Component Name Base Name Common Name External Name
CD19+ B CELLS % CD19PLUSPCT CD19 PLUS PCT CD19+ B cells %
CD19+ B CELLS CD19PLUSABS CD19 PLUS ABS CD19+ B cells
CD20+ % CD20PLUSPCT CD20 PLUS PCT CD20+ %
CD20+ CD20PLUSABS CD20 PLUS ABS CD20+
TOTAL MEMORY CD27+ % MEMCD27PCT TOTAL MEMORY CD27 POS PCT Total Memory CD27+ %
TOTAL MEMORY CD27+ MEMCD27 TOTAL MEMORY CD27 POS Total Memory CD27+
NONS CD27+IGD+IGM+ % CD27IGDIGMPC NONS CD27 POS IGD POS IGM POS PCT NonS CD27+IgD+IgM+ %
NONS CD27+IGD+IGM+ CD27IGDIGM NONS CD27 POS IGD POS IGM POS NonS CD27+IgD+IgM+
CLASSS CD27+IGD-IGM- % CLASSCD27 CLASS CD27 POS IGD IGM Class-switched CD27+IgD-IgM- %
CLASSS CD27+IGD-IGM- CLASSCD27CL CLASS CD27 POS IGD IGM Class-switched CD27+IgD-IgM-
TRANS CD38+IGM+ % CD38IGMPCT TRANS CD38 POS IGM POS PCT Trans CD38+IgM+ %
TRANS CD38+IGM+ CD38IGM TRANS CD38 POS IGM POS Trans CD38+IgM+
PLASMAB CD38+IGM- % CD38IGMPCT PLASMAB CD38 POS IGM NEG PCT PlasmaB CD38+IgM- %
PLASMAB CD38+IGM- CD38IGM PLASMAB CD38 POS IGM NEG PlasmaB CD38+IgM-
ACT CD21LOW CD38- % ACTCD21 ACT CD21LOW CD38 Activated CD21low CD38- %
ACT CD21LOW CD38- ACTCD21 ACT CD21LOW CD38 Act CD21low CD38-

Result Interpretation

Forms

Flow Cytometry LAB13509 build edit