CMV inSIGHT T Cell Immunity

Synonyms

• CMV INSIGHT T CELL IMMUNITY
• CMVTCELL
• LAB12448

Cerner Name

CMV inSIGHT T Cell Immunity

Clinical Info

***   Must be delivered STAT to Lab *** 

URGENT!! Extremely limited sample stability. Samples MUST be received at CFAM by 12:00 PM (noon), Monday to Thursday only. DO NOT draw if the patient arrives after 12:00 PM. Reschedule patient if they arrive after 12 PM. Request STAT pickup by emailing NorthwellLogistics@northwell.edu and copy CoreReferenceDept@northwell.edu for increased awareness
The CMV inSIGHT™ T Cell Immunity Panel measures the strength of T cell responses to Cytomegalovirus (CMV) specific antigens. It evaluates and reports the activity of CD4 and CD8 T cell responses independently. Effective T cell immunity against CMV is a factor in controlling CMV viral latency. CMV can affect patients with weakened immune systems and is a common risk factor in patients following solid organ or hematopoietic stem cell transplant.

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous

Specimen Types

Blood

Specimen Volume

10 mL Whole Blood Dark Green Top Tube (7.5 mL min)

Pediatric Patients: Whole Blood 4 mL min volume.

Container

Dark Green Sodium Heparin Tube

Collection Instructions

URGENT!! Extremely limited sample stability. Samples MUST be received at CFAM by 12:00 PM (noon), Monday to Thursday only. DO NOT draw if the patient arrives after 12:00 PM. Reschedule patient if they arrive after 12 PM. Request STAT pickup by emailing NorthwellLogistics@northwell.edu and copy CoreReferenceDept@northwell.edu for increased awarenes

Transport Instructions

Note: Blood must be drawn Monday through Thursday only.DO NOT SHIP on days when a holiday follows within 2 days of the shipping day.Ship samples priority overnight Monday through Thursday, at ambient temperature on the same day as collection.

Specimen Stability

24 Hours Room Temperature

Methodology

Whole blood is stimulated with SEB, CMV antigens, or left unstimulated as a negative control and incubated at 37ºC. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD3, CD4, CD8, CD69) and intracellular IFN-gamma, and analyzed by flow cytometry. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Days Performed

TAT: 4-5 Days

Performing Laboratories

Viracor-Euofins

CPT

86352 x 4

PDM

235609

Results

Result Interpretation

Forms

• CMV inSIGHT Specimen Collection Instructions