Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors. Limitations This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results,
Specimen Type
Blood
Container
Lavender Top Tube
Collection Instructions
Container/Tube: 2 Lavender top tubes Specimen: 5 mL plasma Transport Temperature: Frozen Collection Collect specimen in two lavender-top (EDTA) tubes or two PPT™ tubes. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or Pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at - 20° C. Do not place samples on dry ice until completely frozen
Transport Instructions
Specimen Stability
6 Hours Room Temperature 2 Years frozen
Methodology
Polymerase chain reaction (PCR) amplification and DNA sequencing.
