Human Papillomavirus (HPV) Molecular Detection with Reflex to Genotyping, Anal-Rectal Build info

Synonyms

• HPV BY PCR - ANAL SWAB
• HPV BY PCR - ANAL SWAB
• HPVANAL
• LAB11782

Short Name

HPV BY PCR - ANAL SWAB

Procedure Master Number

LAB11782

Procedure ID

115815

Clinical Info

Clinical information, together with the physician’s assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.

Specimen Sources

Specimen Types

Container

PreservCyt

Collection Instructions

Container/Tube: Dacron® swab collected in 3 mL Liquid Cytology (PreservCYT®) Preservative (Thin Prep®) -
Specimen: Entire Container
Transport Temperature: Room Temperature

Note: Specimen source is required on request form for processing.

Collection:
To collect an anal-rectal sample, a wetted, non-lubricated Dacron/Polyester swab is used. The Dacron/Polyester swab is inserted about 3 cm (or until resistance is met) into the anal canal past the anal verge, into the rectal vault. This is done without visualization of the anal canal. Firm lateral pressure is applied to the swab handle as it is rotated and slowly moved in and out. Slowly withdraw swab from the anal canal. Swish the swab vigorously in PreservCyt® fluid in the ThinPrep® vial. Discard the swab. Cap and tighten the ThinPrep® vial. Follow the same procedure if using a brush to collect an anal-rectal sample. Avoid using cotton swab on a wooden stick because the handle may break and splinter during collection. Swab that is grossly contaminated with feces should be discarded and the collection repeated.

Transport Instructions

Room Temperature

Specimen Stability

Room temperature: 30 days
Refrigerated: 28 days
Frozen: Unacceptable

Methodology

Transcription-Mediated Amplification (TMA)  
 

Days Performed

TAT 4 - 7 days
The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability.

Performing Laboratory

Quest Diagnostics' Nichols Institute, Inc. - Chantilly

CPT

87624

PDM

Only Orderable at Locations:

Results

Forms

• Cytology Anal Brush With or Without HPV 7-19-17
• Anal Brush Cytology and Anal HPV 2-27-2023