Clinical information, together with the physician’s assessment of cytology history, other risk factors, and professional guidelines, may be used to guide patient management.
Specimen Type
Container
PreservCyt
Collection Instructions
Container/Tube: Dacron® swab collected in 3 mL Liquid Cytology (PreservCYT®) Preservative (Thin Prep®) - Specimen: Entire Container Transport Temperature: Room Temperature
Note: Specimen source is required on request form for processing.
Collection: To collect an anal-rectal sample, a wetted, non-lubricated Dacron/Polyester swab is used. The Dacron/Polyester swab is inserted about 3 cm (or until resistance is met) into the anal canal past the anal verge, into the rectal vault. This is done without visualization of the anal canal. Firm lateral pressure is applied to the swab handle as it is rotated and slowly moved in and out. Slowly withdraw swab from the anal canal. Swish the swab vigorously in PreservCyt® fluid in the ThinPrep® vial. Discard the swab. Cap and tighten the ThinPrep® vial. Follow the same procedure if using a brush to collect an anal-rectal sample. Avoid using cotton swab on a wooden stick because the handle may break and splinter during collection. Swab that is grossly contaminated with feces should be discarded and the collection repeated.
Transport Instructions
Room Temperature
Specimen Stability
Room temperature: 30 days Refrigerated: 28 days Frozen: Unacceptable
Methodology
Transcription-Mediated Amplification (TMA)
Days Performed
TAT 4 - 7 days The presence of E6/E7 messenger RNA from 14 high risk HPV types indicates incorporation of HPV DNA into the host cells. Proteins expressed from E6-E7 polycistronic mRNA alter cellular p53 and retinoblastoma protein functions, leading to disruption of cell-cycle check points and cell genome instability.
Performing Laboratory
Quest Diagnostics' Nichols Institute, Inc. - Chantilly
