T Cell Gene Rearrangement (TCR) Molecular Detection

Synonyms

• LAB11405
• MG TCR
• T CELL GAMMA
• T CELL REARRANGEMENT
• T-CELL RECEPTOR GAMMA REARRANGEMENT
• TCR BY PCR CASE
• TCRG

Cerner Name

HLX TCR by PCR Case

Clinical Info

Leukemias and lymphomas are characterized by the presence of clonal B-or T-cell populations. Rearrangement of the antigen receptor genes occur during the ontogeny of B and T cell lymphocytes. The rearrangements are unique in length and sequence for each cell. The T-cell receptor genes in T-cells are composed of 4 different chains α, β, γ and δ. Each one is the product of rearrangement of the Variable, Diversity, Constant and Joining regions of the respective genes. Each B- and T-cell has a single productive V-J rearrangement that is unique in both length and sequence. 

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous Bone Marrow

Specimen Types

Aspirate Blood

Container

Collection Instructions

Container/Tube: Lavender Top Tube or Sterile Container
Specimen: 1-3 mL of Peripheral Blood or Bone Marrow Aspirate collected in lavender top (EDTA) tube, transport at room temperature or 4°C.
Pleural Fluid (thoracentesis fluid) and fine needle aspirate (FNA) specimens collected in a sterile tube, transport at 4°C, submit at least 0.5 x 10^7 cells.
Formalin fixed paraffin embedded(FFPE) tissue, specimens should be representative of the tumor (contain neoplastic cells) as determined per pathologist.
5-10 Sections, 10 um thick. FFPE tissue can be stored indefinitely at room temperature.
Transport Temperature: Blood or Bone marrow: Room temperature or Refrigerated. Do Not Freeze.        FFPE: Room temperature, FNA and Pleural Fluid: Refrigerated

Specimen Volume

Transport Instructions

room temperature or 4°C

Specimen Stability

Blood or Bone marrow; 7 Days Refrigerated. Do Not Freeze
FFPE Indefinitely at room temperature
FNA and Pleural Fluid 7 Days Refrigerated

Methodology

Multiplex Polymerase Chain Reaction (PCR) Amplification followed by Capillary Electrophoresis Analysis

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

81342

PDM

5160176

Only Orderable at Locations:

Orderable Everywhere

Results

Result Interpretation

Forms