T Cell Gene Rearrangement (TCR) Molecular Detection Build info

Synonyms

  • MOLECULAR GENETICS - TCR
  • MG TCR
  • TCRG
  • T CELL GAMMA
  • T-CELL RECEPTOR GAMMA REARRANGEMENT
  • HLX TCR BY PCR CASE
  • LAB11405

Short Name

MOLECULAR GENETICS - TCR

Procedure Master Number

LAB11405

Procedure ID

115151

Clinical Info

Leukemias and lymphomas are characterized by the presence of clonal B-or T-cell populations. Rearrangement of the antigen receptor genes occur during the ontogeny of B and T cell lymphocytes. The rearrangements are unique in length and sequence for each cell. The T-cell receptor genes in T-cells are composed of 4 different chains α, β, γ and δ. Each one is the product of rearrangement of the Variable, Diversity, Constant and Joining regions of the respective genes. Each B- and T-cell has a single productive V-J rearrangement that is unique in both length and sequence. 

Specimen Type

Blood, Body Fluid, Bone Marrow, Tissue, Other

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender Top Tube or Sterile Container
Specimen: 1-3 mL of Peripheral Blood or Bone Marrow Aspirate collected in lavender top (EDTA) tube, transport at room temperature or 4°C.
Pleural Fluid (thoracentesis fluid) and fine needle aspirate (FNA) specimens collected in a sterile tube, transport at 4°C, submit at least 0.5 x 10^7 cells.
Formalin fixed paraffin embedded(FFPE) tissue, specimens should be representative of the tumor (contain neoplastic cells) as determined per pathologist.
5-10 Sections, 10 um thick. FFPE tissue can be stored indefinitely at room temperature.
Transport Temperature: Blood or Bone marrow: Room temperature or Refrigerated. Do Not Freeze.        FFPE: Room temperature, FNA and Pleural Fluid: Refrigerated

Transport Instructions

room temperature or 4°C

Specimen Stability

Blood or Bone marrow; 7 Days Refrigerated. Do Not Freeze
FFPE Indefinitely at room temperature
FNA and Pleural Fluid 7 Days Refrigerated

Methodology

Multiplex Polymerase Chain Reaction (PCR) Amplification followed by Capillary Electrophoresis Analysis

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

81342

PDM

5160176

Results