FLT3 ITD-TKD Mutation Molecular Detection

Synonyms

• FLT3 CASE
• FLT3 ITD-TKD MUTATION
• FLT3 TKD, FLT3 D835, FLT3 MUTANT, FLT3 ITD
• LAB11399

Cerner Name

HLX FLT3 CASE

Clinical Info

Acute myelogenous leukemia (AML) is a rare but devastating disease resulting in 1.2% of all cancer related deaths. One of the most common genes mutated in AML is
the FMS-Iike tyrosine kinase 3, FLT3. FLT3 is a type Ill tyrosine kinase receptor expressed on the surface of hematopoietic stem cells that
regulates differentiation and proliferation of hematopoietic elements. A point mutation in the activation loop of the tyrosine kinase domain (TKD) or an
internal tandem duplications (ITD) in the juxtamembrane domain cause constitutive activation of the FLT3 receptor.
Studies have shown that AML patients with FLT3-ITD have a lower complete remission rate (78% vs 84%), shorter disease-free survival (30% vs 46% at 5 years)
and lower overall survival rate (33% vs 44%) at 5 years. FLT3-TKD mutation has unclear prognosis.
Assessing the mutation status of FLT3 may impact diagnosis, prognosis and therapy of patients affected with AML. FLT3-ITD or TKD positive patients may be
eligible for therapy with XOSPATA, an FDA-approved drug that inhibits ITD and TKD mutations.  

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous Bone Marrow Bone Marrow Aspirate

Specimen Types

Aspirate Blood Bone Marrow

Specimen Volume

1 - 3 mL peripheral blood or Bone marrow

Container

Collection Instructions

Container/Tube: Lavender top tube (EDTA) 
Specimen: 1 - 3 mL peripheral blood or Bone marrow
Transport Temperature: Room Temperature or 4C

Transport Instructions

Room Temperature or 4C

Specimen Stability

Specimens are stable for a week refrigerated.

Methodology

Polymerase chain reaction (PCR) amplification of FLT3 ITD followed by fragment analysis by capillary electrophoresis, TKD is performed by PCR RFLP (EcorRV) followed by capillary electrophoresis.

Days Performed

Monday through Friday
TAT: 8 Calendar days

Performing Laboratory

Northwell Health Laboratories

CPT

81245
81246

PDM

1863168

Only Orderable at Locations:

Orderable Everywhere

Results

Result Interpretation

Forms