FLT3 ITD-TKD Mutation Molecular Detection Build info

Synonyms	FLT3 ITD MUTATION ANALYSIS HLX FLT3 CASE FLT3 TKD, FLT3 D835, FLT3 MUTANT, FLT3 ITD HLX FLT3 LAB11399
Short Name	FLT3 ITD MUTATION ANALYSIS
Procedure Master Number	LAB11399
Procedure ID	115139
Clinical Info	Acute myelogenous leukemia (AML) is a rare but devastating disease resulting in 1.2% of all cancer related deaths. One of the most common genes mutated in AML is the FMS-Iike tyrosine kinase 3, FLT3. FLT3 is a type Ill tyrosine kinase receptor expressed on the surface of hematopoietic stem cells that regulates differentiation and proliferation of hematopoietic elements. A point mutation in the activation loop of the tyrosine kinase domain (TKD) or an internal tandem duplications (ITD) in the juxtamembrane domain cause constitutive activation of the FLT3 receptor. Studies have shown that AML patients with FLT3-ITD have a lower complete remission rate (78% vs 84%), shorter disease-free survival (30% vs 46% at 5 years) and lower overall survival rate (33% vs 44%) at 5 years. FLT3-TKD mutation has unclear prognosis. Assessing the mutation status of FLT3 may impact diagnosis, prognosis and therapy of patients affected with AML. FLT3-ITD or TKD positive patients may be eligible for therapy with XOSPATA, an FDA-approved drug that inhibits ITD and TKD mutations.
Specimen Type	Blood, Bone Marrow
Container	Lavender Top Tube
Collection Instructions	Container/Tube: Lavender top tube (EDTA) Specimen: 1 - 3 mL peripheral blood or Bone marrow Transport Temperature: Room Temperature or 4C
Transport Instructions	Room Temperature or 4C
Specimen Stability	Specimens are stable for a week refrigerated.
Methodology	Polymerase chain reaction (PCR) amplification of FLT3 ITD followed by fragment analysis by capillary electrophoresis, TKD is performed by PCR RFLP (EcorRV) followed by capillary electrophoresis.
Days Performed	Monday through Friday TAT: 8 Calendar days
Performing Laboratory	Northwell Health Laboratories
CPT	81245 81246
PDM	1863168
Results