BCR/ABL1, Tyrosine Kinase Inhibitor Resistance, Kinase Domain Mutation Screen, Sanger Sequencing

Synonyms

• BCR/ABL1 TKIR SEQUENCING
• LAB11201

Cerner Name

BCR/ABL1 Tyrosine Seq

Clinical Info

Evaluating patients with chronic myelogenous leukemia and Philadelphia chromosome positive B-cell acute lymphoblastic leukemia receiving tyrosine kinase inhibitor (TKI) therapy, who are apparently failing treatment
Preferred initial test to identify the presence of acquired BCR-ABL1 mutations associated with TKI-resistance.

Specimen Sources

Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous Bone Marrow Bone Marrow Aspirate

Specimen Types

Aspirate Blood Bone Marrow

Container

Lavender Top Tube

Collection Instructions

Container/Tube: Lavender(EDTA) Top Tube
Specimen: 10 mL Whole Blood (8 mL min) or 4 mL Bone marrow (2mL min)
Transport temperature: Refrigerated
Note: Refrigerated specimens must arrive within 5 days of collection, and ambient specimens must arrive within 3 days (72 hours) of collection.
The following information is required:
1. Patient's fusion type (p210, p190, p205 or p230)
2. Pertinent clinical history
3. Clinical or morphologic suspicion
4. Specimen source (blood or bone marrow)

Specimen Volume

Transport Instructions

Specimen Stability

3 Days Room Temperature
5 Days Refrigerated
Unacceptable Frozen

Methodology

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with Sanger Sequencing

Days Performed

TAT: 6-8 Days
If BCR/ABL1 fusion type (p210, p190, p205 or p230) is not provided, BADX / BCR/ABL1, Qualitative, Diagnostic Assay, Varies will be performed at an additional charge.
In the event that no fusion form (p190, p205, p210, p230) is identified by qualitative testing, this test will be canceled.

Performing Laboratory

Mayo Medical Laboratories

CPT

81170
LOINC Code: 55135-8

PDM

235768

Only Orderable at Locations:

Orderable Everywhere

Results

Result Interpretation

Forms