CD3, Absolute Count (CAR-T) Build info

Synonyms

• CD3, ABSOLUTE COUNT
• CD3ABS
• LAB11155

Short Name

CD3, ABSOLUTE COUNT

Procedure Master Number

LAB11155

Procedure ID

114694

Clinical Info

This assay measures the absolute count and viability of CD3+ T-cells. It can be used in the following clinical scenarios. First, the CD3 absolute count is measured in stem cell transplant products when it is important that the number of CD3 T cells present is as low as possible (rare event testing), to prevent Graft versus Host disease. Second, CAR-T therapy is a new type of cancer treatment that uses the immune system to kill cancer cells in patients with B cell neoplasms. CAR-T therapy works by modifying a patient’s own T cells and infusing them back into the patient. Third, Donor Lymphocyte Infusion (DLI) is a form of immunotherapy used for patients that have received Matched Unrelated Donor (MUD) transplants. DLIs are used to induce remission of the patient’s disease by a process called ‘graft versus tumour’ effect. The donor T cells will attack and control the growth of the residual malignant cells and lead the patient into remission. The viability of these lymphocytes is measured to determine whether the cells are still living so they can be used for treatment.

Specimen Type

Blood, Other

Container

Lavender Top Tube

Collection Instructions

Peripheral Blood or Apheresis sample. Immediate Delivery to lab is recommended. Refrigerate after 24 hours post collection.

Transport Instructions

Immediate Delivery is recommended.

Specimen Stability

Refrigerate after 24 hours post collection. Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s).
 

Methodology

Flow Cytometry

Days Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays from 8AM-4PM. Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin LutherKing Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Performing Laboratory

Northwell Health Test Laboratories

CPT

86367

PDM

5200246

Results