Flow Cytometry - CD3, Absolute Count (CAR-T)
Test Catalog Information
| Test Catalog Synonyms | |
| EPIC Synonyms | CD3ABS |
| Cerner Primary Mnemonic | CD3, Absolute Count |
| EPIC Display Name | Flow Cytometry - CD3, Absolute Count (CAR-T) |
| Allscripts (AEHR) Order Name | CD3, Absolute Count |
| Sunrise Clinical Manager (SCM) Order Name | CD3, Absolute Count |
| EPIC Inpatient Orderable | Yes |
| EPIC Outpatient Orderable | Yes |
| Cerner Results |
CD3 Viability CD3ABS |
| Clinical Info |
This assay measures the absolute count and viability of CD3+ T-cells. It can be used in the following clinical scenarios. First, the CD3 absolute count is measured in stem cell transplant products when it is important that the number of CD3 T cells present is as low as possible (rare event testing), to prevent Graft versus Host disease. Second, CAR-T therapy is a new type of cancer treatment that uses the immune system to kill cancer cells in patients with B cell neoplasms. CAR-T therapy works by modifying a patient’s own T cells and infusing them back into the patient. Third, Donor Lymphocyte Infusion (DLI) is a form of immunotherapy used for patients that have received Matched Unrelated Donor (MUD) transplants. DLIs are used to induce remission of the patient’s disease by a process called ‘graft versus tumour’ effect. The donor T cells will attack and control the growth of the residual malignant cells and lead the patient into remission. The viability of these lymphocytes is measured to determine whether the cells are still living so they can be used for treatment. |
| Specimen Type |
Blood, Other |
| Container |
Lavender Top Tube |
| Collection Instructions |
Peripheral Blood or Apheresis sample. Immediate Delivery to lab is recommended. Refrigerate after 24 hours post collection. |
| Transport Instructions |
Immediate Delivery is recommended. |
| Specimen Stability |
Refrigerate after 24 hours post collection. Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s). |
| Methodology |
Flow Cytometry |
| Days Performed |
Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays from 8AM-4PM. Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin LutherKing Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day. |
| Performing Laboratory |
Northwell Health Test Laboratories |
| CPT |
86367 |
| PDM |
5200246 |
Desired Epic Build Flow Cytometry - CD3, Absolute Count (CAR-T)
| Cerner Primary Mnemonic: | CD3, Absolute Count | |||||||||
| PDM | 5200246 | |||||||||
| Informatics - Workgroup | Flow Cytometry | |||||||||
| Synonyms * | CD3ABS | |||||||||
| Display Name * | Flow Cytometry - CD3, Absolute Count (CAR-T) | |||||||||
| Order Entry Specimen Sources * | ||||||||||
| Order Entry Specimen Types |
Blood
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| Ordering info (EPIC SmartText) | This assay measures the absolute count and viability of CD3+ T-cells. It can be used in the following clinical scenarios. First, the CD3 absolute count is measured in stem cell transplant products when it is important that the number of CD3 T cells present is as low as possible (rare event testing), to prevent Graft versus Host disease. Second, CAR-T therapy is a new type of cancer treatment that uses the immune system to kill cancer cells in patients with B cell neoplasms. CAR-T therapy works by modifying a patient’s own T cells and infusing them back into the patient. Third, Donor Lymphocyte Infusion (DLI) is a form of immunotherapy used for patients that have received Matched Unrelated Donor (MUD) transplants. DLIs are used to induce remission of the patient’s disease by a process called ‘graft versus tumour’ effect. The donor T cells will attack and control the growth of the residual malignant cells and lead the patient into remission. The viability of these lymphocytes is measured to determine whether the cells are still living so they can be used for treatment. | |||||||||
| IP Orderable | Yes | |||||||||
| OP Orderable | Yes | |||||||||
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AOEs * | ||||||||||
| AP AOEs | ||||||||||
| Special History | No | |||||||||
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| Procedure Category Change | ||||||||||
Cerner Results
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Current Actual EPIC Build as of 10/28/2024
| Procedure Id | 114694 | ||||||||||||||||||
| Pdm | 5200246 | ||||||||||||||||||
| Order Display Name | Flow Cytometry - CD3, Absolute Count (CAR-T) | ||||||||||||||||||
| Procedure Name | CD3, ABSOLUTE COUNT | ||||||||||||||||||
| Procedure Master Number | LAB11155 | ||||||||||||||||||
| Short Procedure Name | CD3, ABSOLUTE COUNT | ||||||||||||||||||
| Category Code | 1.0 | ||||||||||||||||||
| Category Code Record Name | LAB BLOOD ORDERABLES | ||||||||||||||||||
| Synonyms | CD3ABS | ||||||||||||||||||
| Clinically Active | Yes | ||||||||||||||||||
| Orderable | Yes | ||||||||||||||||||
| Performable | Yes | ||||||||||||||||||
| Filter Genomics | |||||||||||||||||||
| Reference Link Url | https://labs.northwell.edu/epic/test/114694 | ||||||||||||||||||
| Ordering Instructions | |||||||||||||||||||
| Default Specimen Type | Blood | ||||||||||||||||||
| Specimen Type Pick List | Blood | ||||||||||||||||||
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| Specimen Source Pick List | Blood, Arterial Blood, Capillary Blood, Central Line Blood, Venous | ||||||||||||||||||
| Specimen Source Default - Male | Blood, Venous | ||||||||||||||||||
| Specimen Source Default - Female | Blood, Venous | ||||||||||||||||||
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| Order Questions | [] | ||||||||||||||||||
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| Edp Ip Specimen Source | Blood, Venous Blood, Central Line Blood, Arterial Blood, Capillary | ||||||||||||||||||
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| Edp Ip Specimen Type | Blood | ||||||||||||||||||
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| Derived Edp Ip Buttons S | Blood, Central Line Blood, Venous Blood, Capillary Blood, Arterial | ||||||||||||||||||
| Derived Edp Ip Buttons T | Blood | ||||||||||||||||||
| Derived Edp Op Buttons S | |||||||||||||||||||
| Derived Edp Op Buttons T | |||||||||||||||||||
| Ip Orderable | 1 | ||||||||||||||||||
| Op Orderable | 1 | ||||||||||||||||||
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EPIC Components (results)
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