Assessing T-cell function in patients on immunosuppressive therapy, including solid-organ transplant patients Evaluating patients suspected of having impairment in cellular immunity Evaluation of T-cell function in patients with primary immunodeficiencies, either cellular (DiGeorge syndrome, T-negative severe combined immunodeficiency: SCID, etc) or combined T- and B-cell immunodeficiencies (T- and B-negative SCID, Wiskott Aldrich syndrome, ataxia telangiectasia, common variable immunodeficiency, among others) where T-cell function may be impaired Evaluation of T-cell function in patients with secondary immunodeficiency, either disease related or iatrogenic Evaluation of recovery of T-cell function and competence following bone marrow transplantation or hematopoietic stem cell transplantation
Specimen Sources
Blood, ArterialBlood, CapillaryBlood, Central LineBlood, Venous
Specimen Types
Blood
Container
Green Top Tube
Collection Instructions
NOTE: DO NOT DRAW FRIDAY, SATURDAY OR SUNDAY Container/Tube: Dark Green (sodium heparin) Top tube Specimen: Whole Blood <3 months: 1 mL 3 months-5 years: 2 mL 6-18 years: 3 mL >18 years: 10 mL Minimum volume 1 mL Collection Instructions: Send specimen is original tube. Do not aliquot. Transport Temperature: Room Temperature ONLY Stability: 48 Hours Room Temperature
Specimen Volume
Whole Blood
Transport Instructions
Room Temperature ONLY
Specimen Stability
48 Hours Room Temperature
Methodology
Flow Cytometry
Days Performed
Monday through Friday
Do not send specimen after Thursday.
Performing Laboratory
Mayo Medical Laboratories NOTE: DO NOT DRAW FRIDAY, SATURDAY OR SUNDAY
CPT
86353 86353 (if appropriate) LOINC Code: 69042-0
PDM
5901650
Only Orderable at Locations:
Orderable Everywhere
Results
Component Name
Base Name
Common Name
External Name
LYMPH ANTIGEN/MITOGEN PROLIFE
LYMPHAG
LYMPH ANTIGEN MITOGEN PROLIFE
Lymph Antigen/Mitogen Prolife
Result Interpretation
Viability of lymphocytes at day 0: ≥75.0%
Maximum proliferation of Candida albicans as % CD45: ≥5.7%
Maximum proliferation of Candida albicans as % CD3: ≥3.0%
Maximum proliferation of tetanus toxoid as % CD45: ≥5.2%