NMR LDL-P is a management tool used in appropriate high-risk patients (type 2 diabetes mellitus, metabolic syndrome, CVD risk equivalent, statin-treated patients) to adjudicate response to treatment and guide adjustment in therapy. It is used in conjunction with other lipid measurements and clinical evaluation to aid in the management of lipoprotein disorders associated with cardiovascular disease.
Specimen Sources
Blood, ArterialBlood, CapillaryBlood, Central LineBlood, Venous
Specimen Types
Blood
Specimen Volume
2 mL serum ( 1 mL min)
Container
Serum, NMR LipoTube (black-and-yellow-top, gel-barrier), preferred; or serum, plain (red-top, no gel); or plasma, EDTA (lavender-top, no gel); keep refrigerated and ship with frozen cool packs
Collection Instructions
Serum, NMR LipoTube (black-and-yellow-top, gel-barrier), preferred; or serum, plain (red-top, no gel); or plasma, EDTA (lavender-top, no gel); keep refrigerated and ship with frozen cool packs Patient Preparation Patient should be fasting for 12 to 14 hours.
Collection Instructions Collect specimen in NMR LipoTube (black-and-yellow-top, gel-barrier),which is the preferred tube. Keep upright at room temperature for 30 minutes and allow to clot. Immediately after clotting, centrifuge at 1600 to 1800 xg for 10 to 15 minutes. The NMR LipoTube should then be stored at 2°C to 8°C until shipped. Do not open NMR LipoTube.
For plain serum (red-top, no gel), keep upright at room temperature for 45 minutes and allow to clot. Centrifuge immediately after clotting. Separate serum and transfer to a plastic transport tube. The serum should then be stored at 2°C to 8°C until shipped.
For EDTA plasma (lavender-top, no gel), invert tube several times to mix contents and centrifuge immediately after collection. Separate plasma and transfer to a plastic transport tube. The plasma should then be stored at 2°C to 8°C until shipped.
Serum or plasma drawn in gel-barrier collection tubes other than the NMR LipoTube should not be used.
Transport Instructions
Refrigerated
Specimen Stability
Refrigerated: 14 days Frozen: 14 days
Methodology
Nuclear magnetic resonance (NMR)
This test includes historical reporting (LDL-P, LDL-C); insulin-resistance score; lipoprotein particle number (LDL-P); particle concentration and size (total HDL-P, small LDL-P, LDL size); and standard lipid panel (total cholesterol, calculated LDL cholesterol, HDL cholesterol, triglycerides).
