Lymphocyte Subsets: T cells (CD3/4/8) Build info

Synonyms

  • T CELL SUBSET
  • CD3
  • T-CELL
  • CD8
  • CD4
  • THS
  • LAB10552

Short Name

T CELL SUBSET

Procedure Master Number

LAB10552

Procedure ID

111608

Clinical Info

This assay provides absolute cell counts and percentages for CD3+ T-cells, CD4 and CD8 T cell subsets,as well as CD4/CD8 ratio.

Specimen Type

Blood

Container

EDTA (Lavender) or Sodium Heparin (Green top)

Collection Instructions

Specimen must arrive within 12 hours of draw. Contact the Flow Cytometry Laboratory at CFAM at CFAMFlow@northwell.edu for collection instructions and consultation on selection of test(s). Container/Tube: Lavender-top (EDTA) tube or Green (Sodium Heparin) Tube Specimen:  3 mL of whole blood Transport Temperature:  Ambient Collection Instructions:  Forward promptly. Specimen cannot be refrigerated. Rejection Criteria - All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 24 hours post collection and the sample has not been preserved, unless viability is greater than 50%.

Transport Instructions

Ambient

Specimen Stability

Delivery to the lab within 12 hours is recommended

Methodology

Flow Cytometry includes CD3, CD4, CD8, ABS CD3, ABS CD4, ABS CD8 and 4/8 Ratio

Days Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Limited testing is available on Saturdays from 8AM-4PM. 
Laboratory is closed on Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Performing Laboratory

Northwell Core Lab at CFAM

CPT

86359,86360

PDM

5201208

Results

Component Name Base Name Common Name External Name
4/8 RATIO 48RATIO 4 8 RATIO 4/8 Ratio
ABS CD3 CD3TABS CD3 ABS CD3 Abs
ABS CD4 CD4ABS CD4 ABS Abs Cd4
ABS CD8 CD8ABS CD8 ABS Abs Cd8
CD3 % CD3PCT CD3 PCT Cd3 %
CD4 % CD4PCT CD4 PCT Cd4 %
CD8 % CD8PCT CD8 PCT Cd8 %
VIABILITY VIABILITY VIABILITY Viability